PXD073890-1

PXD073890 is an original dataset announced via ProteomeXchange.

Title	TurboID Proximity Profiling of FIKK10.2 (PF3D7_1039000) in Plasmodium falciparum–Infected Red Blood Cells During Febrile Heat Stress
Description	This project investigates how febrile heat stress alters the local protein environment of the Maurer’s cleft resident kinase FIKK10.2 in the malaria parasite Plasmodium falciparum. During human infection, parasites are routinely exposed to elevated temperatures associated with fever, yet how this physiological stress affects the organisation and trafficking of exported proteins within infected red blood cells remains poorly understood. To address this, we used proximity-dependent biotinylation (TurboID) to map changes in the protein neighbourhood of FIKK10.2 under normal and heat stress conditions. Transgenic parasites were generated expressing TurboID fused to the C-terminus of endogenous FIKK10.2. Highly synchronous (two hours window) asexual blood-stage parasites were exposed to febrile heat stress (39 °C) and compared with parasites maintained at standard culture temperature (37 °C). Biotin labelling was applied either continuously throughout the asexual life cycle or as a defined pulse between 16–24 hours post-invasion, with or without heat stress, resulting in three experimental conditions. Infected red blood cells were harvested at 40 hours post-invasion following Percoll density enrichment and lysed in 8 M urea. Proteins were digested to peptides, and biotinylated peptides were selectively enriched using anti-biotin antibodies. Enriched peptides were eluted under acidic conditions and analysed by liquid chromatography–tandem mass spectrometry. The resulting dataset provides a high-resolution view of the FIKK10.2 proximal proteome and, by extension, the Maurer’s cleft protein environment. These data reveal how febrile heat stress influences protein trafficking and local protein composition in P. falciparum, offering insights into temperature-dependent regulation of host cell remodelling during malaria infection.
HostingRepository	PRIDE
AnnounceDate	2026-05-14
AnnouncementXML	Submission_2026-05-13_22:51:04.848.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Jones
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Plasmodium falciparum (isolate 3D7); NCBI TaxID: NEWT:36329;
ModificationList	biotinylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-01 13:33:26	ID requested
⏵ 1	2026-05-13 22:51:05	announced

10.7554/eLife.107860;

Jones D, Belda H, Broncel M, Fuchs G, Anaguano D, Nofal SD, Treeck M, surface proteins into the red blood cell. Elife, 14():(2026) [pubmed]

submitter keyword: Proximity Labelling, Heat stress,TurboID, Plasmodium falciparum

Moritz Treeck
contact affiliation	Gulbenkian Institute for Molecular Medicine (GIMM), Lisbon, Portugal
contact email	moritz.treeck@gimm.pt
lab head
David Jones
contact affiliation	The Francis Crick Institute
contact email	david.a.e.jones@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073890

PRIDE project URI

• PRIDE
  1. PXD073890
     1. Label: PRIDE project
     2. Name: TurboID Proximity Profiling of FIKK10.2 (PF3D7_1039000) in Plasmodium falciparum–Infected Red Blood Cells During Febrile Heat Stress