PXD073884-1

PXD073884 is an original dataset announced via ProteomeXchange.

Title	Proteomic studies of es incorporation in S. cattleya WT and ΔbesG strains.
Description	With in vitro biochemical studies showing that βes can be mistranslated for Thr and selectively edited by BesG, we sought to evaluate the ability of BesG to prevent in vivo misincorporation of βes in the native producer S. cattleya. We therefore disrupted the besG gene in S. cattleya and supplied βes externally to WT S. cattleya and S. cattleya ΔbesG strains through the addition of spent media during exponential growth (Extended Data Figure 2). The proteomes were extracted and derivatized using the copper-catalyzed azide-alkyne cycloaddition (CuAAC) to a TAMRA fluorophore to label detection of βes incorporation by fluorescence. The fluorescence of the ΔbesG knockout strain was notably higher than WT, demonstrating that the absence of BesG appears to increase mistranslation at physiological concentrations of βes (Fig. 2D). Additional proteomics studies confirm that βes is found at Thr sites in the S. cattleya ΔbesG knockout strain (Table S2, Fig. S5).
HostingRepository	PRIDE
AnnounceDate	2026-06-08
AnnouncementXML	Submission_2026-06-08_01:25:16.428.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Douglas Millar
SpeciesList	scientific name: Streptantibioticus cattleyicolor; NCBI TaxID: NEWT:29303;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-31 21:45:02	ID requested
⏵ 1	2026-06-08 01:25:17	announced

10.1038/s41557-026-02126-5;

Millar DC, Zhou Y, Marchand JA, Roberts-Mataric H, Chen T, Hu CX, Fang J, Millar S, Stone EA, Pelton JG, Sosa MB, Chang MCY, tRNA-deacylase-directed discovery of biosynthetic pathways. Nat Chem, 18(5):863-871(2026) [pubmed]

submitter keyword: S. cattleya, beta-ethynylserine

Douglas Millar
contact affiliation	UC-Berkeley
contact email	dcmillar@berkeley.edu
lab head
Douglas Millar
contact affiliation	University of California, Berkeley
contact email	millardouglasc@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD073884
     1. Label: PRIDE project
     2. Name: Proteomic studies of es incorporation in S. cattleya WT and ΔbesG strains.