PXD073871-1

PXD073871 is an original dataset announced via ProteomeXchange.

Title	Laboratory and in-situ insights into the chemoautotrophic and zero-valent sulfur formation pathway in a cold-seep bacterium
Description	Microbial sulfur cycling and chemoautotrophy are focal points of research in cold seeps. However, limited culture-dependent and in-situ studies have described the biological features and ecological significance of chemoautotrophic sulfur-oxidizing bacteria. In this study, we isolated Guyparkeria hydrothermalis SP2, a thiosulfate/sulfide-oxidizing chemoautotrophic bacterium, from cold-seep sediment. Electron microscopy, Raman spectroscopy, and stoichiometry confirmed the efficient production of zero-valent sulfur (ZVS) by G. hydrothermalis SP2. Genomic, transcriptomic, and qRT-PCR analyses revealed its utilization of the Sox pathway for thiosulfate oxidation and the fccB gene for sulfide oxidation. Its chemoautotrophic capability mediated by the Calvin-Benson-Bassham (CBB) cycle was identified through isotopic and qRT-PCR analyses. In-situ studies demonstrated its ability to produce ZVS by oxidizing sulfide in cold seeps, with a preference for different genes compared to those under laboratory conditions. Metagenomic and metatranscriptomic analyses indicated the ubiquity of its sulfur oxidation-based chemoautotrophic pathway in cold seep sediments. Therefore, this strain holds significance for investigating sulfur oxidation-based chemoautotrophic pathways in cold seeps.
HostingRepository	PRIDE
AnnounceDate	2026-02-25
AnnouncementXML	Submission_2026-02-25_06:26:05.427.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ruining Cai
SpeciesList	scientific name: Guyparkeria hydrothermalis; NCBI TaxID: NEWT:923;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-31 02:08:49	ID requested
⏵ 1	2026-02-25 06:26:05	announced

Dataset with its publication pending

submitter keyword: Cold seep

zero-valent sulfur

chemoautotrophy

Ruining Cai
contact affiliation	Institute of Oceanology Chinese Academy of Sciences
contact email	RuiningCAI@163.com
lab head
Ruining Cai
contact affiliation	Institute of Oceanology Chinese Academy of Sciences
contact email	1651309629@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD073871
     1. Label: PRIDE project
     2. Name: Laboratory and in-situ insights into the chemoautotrophic and zero-valent sulfur formation pathway in a cold-seep bacterium