PXD073864-1

PXD073864 is an original dataset announced via ProteomeXchange.

Title	LC–MS/MS Proteomic Profiling of Apis mellifera Venoms
Description	This project presents a comparative proteomic analysis of crude bee venom collected from geographically and genetically distinct Apis mellifera populations from Türkiye and Cyprus. Bottom-up proteomics was performed using nanoLC–MS/MS on a high-resolution Orbitrap platform operating in data-dependent acquisition (DDA) mode. The study aimed to characterize regional differences in venom composition and to investigate potential genetic and post-translational variability in melittin, the dominant venom peptide. Proteomic profiling revealed that melittin is the predominant venom component across all populations, while regional discrimination is primarily driven by coordinated variation in moderately and low-abundance proteins, including phospholipase A2 (PLA2), apamin, secapin family peptides, and hyaluronidase. Targeted re-analysis of MS/MS spectra identified trace but reproducible O-linked glycosylation on melittin, representing the first evidence of naturally occurring O-glycosylation in this peptide. Label-free quantification was applied to assess relative protein abundances and glycoform distribution across regions.
HostingRepository	PRIDE
AnnounceDate	2026-02-02
AnnouncementXML	Submission_2026-02-02_02:03:32.808.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	HACI MEHMET KAYILI
SpeciesList	scientific name: Apis mellifera (Honeybee); NCBI TaxID: NEWT:7460;
ModificationList	complex glycosylation
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-30 13:26:18	ID requested
⏵ 1	2026-02-02 02:03:33	announced

Dataset with its publication pending

submitter keyword: None

Hacı Mehmet
contact affiliation	Faculty of Engineering and Natural Science, Biomedical Engineering Department, Karabuk University, Karabuk, Türkiye
contact email	h.mehmetkayili@karabuk.edu.tr
lab head
HACI MEHMET KAYILI
contact affiliation	Karabuk University Biomedical Engineering Department
contact email	h.mehmetkayili@karabuk.edu.tr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD073864

PRIDE project URI

• PRIDE
  1. PXD073864
     1. Label: PRIDE project
     2. Name: LC–MS/MS Proteomic Profiling of Apis mellifera Venoms