PXD073831-1

PXD073831 is an original dataset announced via ProteomeXchange.

Title	Identification of the Mitochondrial USP30-Flag Interacting Proteome
Description	Based on our research on the regulation of mitochondrial quality control and the functional mechanisms of the deubiquitinase USP30; here, we utilized HeLa cells stably expressing USP30-Flag to systematically identify its interacting proteome through affinity purification-mass spectrometry (AP-MS). This screening successfully enriched a series of high-confidence proteins that potentially interact with USP30 within the mitochondrial environment. The experimental verification of candidate proteins through co-immunoprecipitation highlighted the robust capability of this AP-MS strategy in capturing functional interactors, providing a molecular basis for further understanding USP30-mediated regulation of mitochondrial proteostasis.
HostingRepository	iProX
AnnounceDate	2026-01-30
AnnouncementXML	Submission_2026-01-30_01:47:18.032.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qinhong Luo
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-30 01:46:49	ID requested
⏵ 1	2026-01-30 01:47:18	announced

Dataset with its publication pending

submitter keyword: USP30, PPI

Zigang Li
contact affiliation	Peking University Shenzhen Graduate School
contact email	lizg@pkusz.edu.cn
lab head
Qinhong Luo
contact affiliation	Peking University Shenzhen Graduate School
contact email	qinhongluo@pku.edu.cn
dataset submitter

iProX dataset URI