PXD073787-1

PXD073787 is an original dataset announced via ProteomeXchange.

Title	North vs. South: Ecoregion-Specific Variations of Venom Toxin Profiles and Functional Activities in Nigerian Bitis arietans and Naja nigricollis
Description	Snakebite envenoming is a major yet neglected tropical disease in sub-Saharan Africa, where antivenom efficacy is critically limited by intraspecific venom variation shaped by local'eco-logical pressures. Nigeria’s sharply contrasting Sudan Savanna (North) and Lowland Rainforest (South) provide an ideal natural system toinvestigate this variation, yet a comparative analysis of its medically important snakes has been lacking. We conducted an integrated proteomic and func-tional characterization of venoms from the puff adder (Bitis arietans) and black-necked spitting cobra (Naja nigricollis) collected in Kaduna (North) and Ibadan (South).Using high-resolution LC-MS/MS, SDS-PAGE, and biochemical assays (PLA₂, protease, fibrinogenolytic, haemolytic, and coagulation activities), we mapped region-specific venom compositions and corresponding toxic activities. Bitis arietans displayed striking ecoregion-driven divergence: southern populations were enriched in ser-ine proteases (27.54%), whereas northern venoms were dominated by lectins (25.4%) and distinct SVMP isoforms. Naja nigricollis showed a conserved PLA₂/3FTx backbone, yet southern venoms ex-hibited elevated SVMP III and LAAO abundance. These molecular differences manifested function-ally, with southern B. arietans showing significantly higher protease activity and southern N.nigricollis exhibiting enhanced PLA₂ activity. Principal component analysis suggested geography as a primary determinant of venom phenotype in both species. This work provides the first integrated proteomic and functional comparison of venoms from northern and southern Nigerian populations of Bitis arietans and Naja nigricollis. While based on a limited number of individuals, the observed differences reveal consistent regional trends that shouldbe interpreted as preliminary and hypoth-esis-generating rather than definitive population-level characteristics.
HostingRepository	PRIDE
AnnounceDate	2026-06-01
AnnouncementXML	Submission_2026-05-31_16:08:24.121.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Crasset
SpeciesList	scientific name: Naja nigricollis; NCBI TaxID: NEWT:8654; scientific name: Bitis arietans; NCBI TaxID: NEWT:8692;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	timsTOF SCP

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-29 09:23:01	ID requested
⏵ 1	2026-05-31 16:08:24	announced

10.3390/toxins18050221;

Adeyi AO, Emmanuel OS, Itang SI, Ajisebiola BS, Kumar M, Rudresha GV, Gond PG, Crasset T, Redureau D, Amorim FG, Sunagar K, Quinton L, . Toxins (Basel), 18(5):(2026) [pubmed]

submitter keyword: Venom Variation

Bitis arietans

Naja nigricollis

Snakebite envenoming

Geo-graphical Venom Phenotypes

Quinton Loïc
contact affiliation	Laboratory of Mass Spectrometry, MolSys Research Unit, University of Liège, 4000 Liège, Belgium
contact email	Loic.Quinton@uliege.be
lab head
Thomas Crasset
contact affiliation	Mass Spectrometry Laboratory - University of Liège
contact email	thomas.crasset@uliege.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD073787

PRIDE project URI

• PRIDE
  1. PXD073787
     1. Label: PRIDE project
     2. Name: North vs. South: Ecoregion-Specific Variations of Venom Toxin Profiles and Functional Activities in Nigerian Bitis arietans and Naja nigricollis