PXD073786-1

PXD073786 is an original dataset announced via ProteomeXchange.

Title	Label-free quantitative proteomics of extracellular vesicles from BT549 cells following 3-day reversine treatment Label-free quantitative proteomics of extracellular vesicles from BT549 cells following 3-day reversine treatment
Description	Chromosomal instability promotes cell migration and invasion via EFEMP1 secretion into extracellular vesicles Triple-negative breast cancer (TNBC) is characterised by high rates of chromosomal instability (CIN) and rewired intercellular communication driven by both soluble factors and extracellular vesicles (EVs). To assess how CIN might affect EV-mediated signalling in TNBC, we studied the EV landscape of TNBC cell lines with induced CIN. We find that CIN leads to increased secretion of EVs and that these EVs promote cell migration of recipient cells. EVs are enriched for extracellular matrix (ECM) proteins, including EFEMP1. Indeed, modulation of EFEMP1 levels in EVs significantly alters migration behaviour of EV-treated cells. We show that EFEMP1 expression is regulated by STAT1, and that EVs from STAT1-deficient cells no longer promote migration, which can be rescued by overexpression of EFEMP1 in STAT1-null cells. Xenografting TNBC cells with EFEMP1 enriched cells promotes migration in zebrafish embryos, suggesting that EFEMP1 expression is a factor that promotes metastasis. Together, our results identify a CIN-associated EV program in TNBC and highlight EFEMP1 as a potential therapeutic target to impair EV-driven tumour cell migration.
HostingRepository	PRIDE
AnnounceDate	2026-03-10
AnnouncementXML	Submission_2026-03-10_09:03:56.551.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Justina Clarinda Wolters
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-29 08:53:19	ID requested
⏵ 1	2026-03-10 09:03:57	announced

Dataset with its publication pending

submitter keyword: BT549

triple-negative breast cancer

reversine

chromosomal instability

extracellular vesicles

exosomes

label-free quantification

LC-MS/MS

Q Exactive Plus

JC Wolters
contact affiliation	Department of Pediatrics, University Medical Center Groningen, University of Groningen, The Netherlands
contact email	j.c.wolters@ucmg.nl
lab head
Justina Clarinda Wolters
contact affiliation	UMCG
contact email	j.c.wolters@umcg.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD073786

PRIDE project URI

• PRIDE
  1. PXD073786
     1. Label: PRIDE project
     2. Name: Label-free quantitative proteomics of extracellular vesicles from BT549 cells following 3-day reversine treatment Label-free quantitative proteomics of extracellular vesicles from BT549 cells following 3-day reversine treatment