PXD073748-1

PXD073748 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic analysis of the zebrafish (Danio rerio) perivitelline fluid; identifying putative cortical alveoli-associated proteins
Description	Upon fertilisation, the egg must have the resources to facilitate successful fertilisation and regulate zygotic growth prior to the activation of the embryonic genome. A key biomarker of egg activation is the synchronised release of of cortical alveoli (CA) from the egg’s cortex into the perivitelline space. Teleost CA are primarily associated with their highly conserved roles in polyspermy prevention. However, several CA-associated proteins with functions related to innate immunity have been isolated. While there is growing evidence that teleost CA have extended biological roles outside of polyspermy prevention, little work has been done towards developing an understanding of their general proteomic composition. Therefore, the aim of this study was to identify proteins which were proportionally over-represented in the zebrafish (Danio rerio) perivitelline fluid (PVF) directly following CA exocytosis to identify a candidate list of CA-associated proteins. This study utilised a novel technique for PVF extraction from water-activated eggs shortly following CA exocytosis which negated the use of fixatives, thereby increasing PVF sample integrity for downstream mass-spectrometry analysis. Moreover, this study provides the first comparative proteomic analysis of the PVF relative to the yolk and cytoplasm of the zebrafish egg. As a result, 44 proteins were identified which were proportionally over-represented in the PVF, relative to the yolk and cytosol, of the water-activated unfertilised egg. The resulting PVF proteome was comprised of proteins associated with functions in carbohydrate binding and peptidase regulation. Many of these proteins are compelling candidates for being CA-derived and have been previously implicated in innate immunity.
HostingRepository	PRIDE
AnnounceDate	2026-04-27
AnnouncementXML	Submission_2026-04-26_16:11:02.434.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Blake Lewis
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: NEWT:7955;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-28 23:03:30	ID requested
⏵ 1	2026-04-26 16:11:03	announced

10.1093/biolre/ioag012;

Lewis BA, Reader KL, Pankhurst MW, Beck CW, Lokman MP, Comparative proteomic analysis of the zebrafish (Danio rerio) perivitelline fluid

identifying putative cortical alveoli-associated proteins. Biol Reprod, 114(4):1312-1323(2026) [pubmed]

submitter keyword: proteomics, zebrafish, perivitelline fluid,cortical alveoli

Mark Lokman
contact affiliation	University of Otago. Department of Zoology.
contact email	mark.lokman@otago.ac.nz
lab head
Blake Lewis
contact affiliation	University of Otago
contact email	blake.lewis@otago.ac.nz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD073748
     1. Label: PRIDE project
     2. Name: Comparative proteomic analysis of the zebrafish (Danio rerio) perivitelline fluid; identifying putative cortical alveoli-associated proteins