PXD073710 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic profiling of dysferlin microdeletion–induced skeletal muscle remodeling in a knock-in mouse model |
| Description | This project examines the proteomic impact of dysferlin deficiency using a genetically defined knock-in mouse model carrying a five-nucleotide microdeletion in the Dysf gene that corresponds to a pathogenic variant identified in limb-girdle muscular dystrophy type R2 (LGMD-R2) patients. Dysferlin is essential for sarcolemmal membrane repair in skeletal muscle, and its loss leads to progressive muscle degeneration associated with inflammation and tissue remodeling. Despite existing dysferlin-deficient mouse models, variant-specific proteomic characterization in vivo remains limited. Skeletal muscle tissues from wild-type and Dysf Δ5/Δ5 mice were analyzed by global, label-free proteomics to define molecular pathways associated with dysferlin loss. Proteins were extracted from muscle lysates, digested using an S-Trap–based workflow, and analyzed by high-resolution nanoLC–MS/MS. Data were acquired in data-independent acquisition mode using dia-PASEF on a trapped ion mobility mass spectrometer, enabling reproducible and comprehensive protein quantification. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-09 |
| AnnouncementXML | Submission_2026-04-09_06:55:53.149.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Wei-Chi Ku |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Bruker Daltonics timsTOF series |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-01-28 05:30:49 | ID requested | |
| ⏵ 1 | 2026-04-09 06:55:55 | announced | |
Publication List
| 10.1038/s41598-026-46635-2; |
| Chen YL, Lin WN, Pan PY, Ku WC, Wang P, Yip PK, Tsui KC, Wu YN, Lin YH, Generation of a novel Dysferlin microdeletion knock-in mouse model mimicking muscular dystrophy-like pathology. Sci Rep, 16(1):(2026) [pubmed] |
Keyword List
| submitter keyword: dysferlinopathy |
| limb-girdle muscular dystrophy |
| skeletal muscle proteomics |
| DIA-PASEF mass spectrometry |
Contact List
| Wei-Chi Ku |
|---|
| contact affiliation | School of Medicine, College of Medicine, Fu Jen Catholic Univeristy, New Taipei City, Taiwan |
| contact email | 089052@mail.fju.edu.tw |
| lab head | |
| Wei-Chi Ku |
|---|
| contact affiliation | Fu Jen Catholic University |
| contact email | 089052@mail.fju.edu.tw |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD073710 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD073710
- Label: PRIDE project
- Name: Proteomic profiling of dysferlin microdeletion–induced skeletal muscle remodeling in a knock-in mouse model
