PXD073709 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Quantitative and Site-Specific Profiling of Protein O-GlcNAcylation in Platinum-Based Chemotherapeutics |
| Description | Platinum (Pt)-based chemotherapeutics is emerging as a promising strategy for cancer treatment in clinical trials. Protein O-linked β-N-acetylglucosamine (O-GlcNAc) modification has been reported to occur on several important proteins implicated in regulating cancer resistance to Pt drugs. However, the O-GlcNAc proteomic landscape during this process remains poorly characterized. Herein, we report quantitative profiling of O-GlcNAcylation sites with carboplatin exposure, by using a chemoenzymatic labeling-assisted chemoproteomic approach. The stoichiometric differences of 169 O-GlcNAcylated sites are quantified, many of which occur on essential genome stability regulators. Furthermore, we discover that the cellular O-GlcNAc level is elevated upon carboplatin treatment and suppression of O-GlcNAcylation sensitizes cancer cells to carboplatin-induced cytotoxicity. These results establish a valuable resource for elucidating the function role of O-GlcNAcylation in drug resistance and shed new light on cancer chemotherapy. The chemoproteomic strategy should be generally applicable for monitoring O-GlcNAc dynamic changes in various pharmacological processes. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-25 |
| AnnouncementXML | Submission_2026-06-24_23:42:49.762.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Qingsong Xie |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | LTQ Orbitrap Elite |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-01-28 05:25:54 | ID requested | |
| ⏵ 1 | 2026-06-24 23:42:50 | announced | |
Publication List
Keyword List
| submitter keyword: O-linked β-N acetylglucosamine (O-GlcNAc),Cancer, LC-MS/MS |
Contact List
| Yi Hao |
|---|
| contact affiliation | National Glycoengineering Research Center, Shandong University, Qingdao 266237, Shandong, China. |
| contact email | yihao@sdu.edu.cn |
| lab head | |
| Qingsong Xie |
|---|
| contact affiliation | Shandong |
| contact email | 202320520@mail.sdu.edu.cn |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD073709
- Label: PRIDE project
- Name: Quantitative and Site-Specific Profiling of Protein O-GlcNAcylation in Platinum-Based Chemotherapeutics
