PXD073685-1

PXD073685 is an original dataset announced via ProteomeXchange.

Title	Synaptic vesicles that store monoamines and glutamate differ in protein composition
Description	Neuromodulators such as monoamines assure essential brain functions but relatively little is known about their mechanism of release. While recent work has advanced our knowledge of active zone architecture in dopamine axons, release machinery within the synaptic vesicle (SV) remains poorly understood. Here, we address differences between the release of monoamines and classical synaptic transmitters by comparing the composition of SVs that contain the vesicular monoamine transporter 2 (VMAT2) versus vesicular glutamate transporter 2 (VGLUT2). Previous work revealed that these SV populations differ in frequency dependence, recycling kinetics and biogenesis. We now find differences in the abundance and isoform expression of many SV protein families. These findings provide new insights into the molecular diversity of SVs and mechanisms of dopamine release.
HostingRepository	PRIDE
AnnounceDate	2026-06-08
AnnouncementXML	Submission_2026-06-08_12:10:36.858.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juan Oses-Prieto
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse; Orbitrap Fusion Lumos; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-27 19:12:46	ID requested
⏵ 1	2026-06-08 12:10:37	announced

Dataset with its publication pending

submitter keyword: synaptic vesicles, TMT,VMAT2, dopamine release, VGLUT2

Kätlin Silm
contact affiliation	Center for Neural Science and Medicine, Board of Governors Regenerative Medicine Institute, Departments of Biomedical Sciences and Neurology, Cedars-Sinai Medical Center, Los Angeles, CA, USA
contact email	Katlin.silm@cshs.org
lab head
Juan Oses-Prieto
contact affiliation	UCSF
contact email	joses@cgl.ucsf.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD073685

PRIDE project URI

• PRIDE
  1. PXD073685
     1. Label: PRIDE project
     2. Name: Synaptic vesicles that store monoamines and glutamate differ in protein composition