PXD073622-1

PXD073622 is an original dataset announced via ProteomeXchange.

Title	Immunoprecipitation Mass Spectrometry Screening of Host Proteins Interacting with RSV M2-1
Description	The immunoprecipitation combined with mass spectrometry (IP-MS) technique was employed to systematically identify the host proteins that physically interact with the RSV M2-1 protein. To achieve reliable detection, the M2-1 protein with a Myc tag was transiently overexpressed in human embryonic kidney 293T cells, and then anti-tag immunoprecipitation was performed under natural conditions. Subsequently, the host proteins co-precipitated were identified and quantified using high-resolution liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). This unbiased proteomic screening revealed the comprehensive interactions between M2-1 and host factors. These findings provided mechanistic insights into the directed regulation of RSV replication by the host and pointed out potential host-dependent factors for therapeutic intervention against RSV infection.
HostingRepository	iProX
AnnounceDate	2026-01-27
AnnouncementXML	Submission_2026-05-20_01:45:08.751.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yufeng Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-27 00:22:47	ID requested
⏵ 1	2026-05-20 01:45:09	announced

Dataset with its publication pending

submitter keyword: Respiratory Syncytial Virus，M2-1 protein，Host protein interactions，Immunoprecipitation Mass Spectrometry

Chuangli Hao
contact affiliation	Children's Hospital of Soochow University
contact email	hcl_md@sina.com
lab head
Yufeng Wang
contact affiliation	Children's Hospital of Soochow University
contact email	18437915003@163.com
dataset submitter

iProX dataset URI