PXD073606 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Harnessing FBXO31 with terminal amide-functionalized molecules for targeted protein degradation |
| Description | Targeted protein degradation (TPD) is a powerful strategy for controlling protein abundance. Here, we establish FBXO31 as a TPD-competent E3 ligase by exploiting its recognition of C-terminal amide-bearing degrons. Using an amidated Ala-Phe motif as a chemical recruiter, multiple small-molecule binders can be transformed into FBXO31-dependent degraders that induce rapid and potent target degradation. Mechanistic studies confirm FBXO31-mediated ternary complex formation and identify key residues in FBXO31 required for recruiter engagement and target degradation. We further show that an FBXO31-based multi-kinase degrader exhibits a distinct and broader degradation profile than a CRBN-based degrader, highlighting a potentially expanded degradable target space beyond CRBN. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-09 |
| AnnouncementXML | Submission_2026-04-09_10:25:43.271.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Xiaoyu Zhang |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | TMT6plex-126 reporter+balance reagent acylated residue |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-01-26 17:42:22 | ID requested | |
| ⏵ 1 | 2026-04-09 10:25:43 | announced | |
Publication List
Keyword List
| submitter keyword: E3 ligase, Targeted protein degradation, Proteomics |
Contact List
| Xiaoyu Zhang |
|---|
| contact affiliation | Northwestern University, Department of Chemistry |
| contact email | zhang@northwestern.edu |
| lab head | |
| Xiaoyu Zhang |
|---|
| contact affiliation | Northwestern University |
| contact email | zhang@northwestern.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD073606 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD073606
- Label: PRIDE project
- Name: Harnessing FBXO31 with terminal amide-functionalized molecules for targeted protein degradation
