PXD073582-1

PXD073582 is an original dataset announced via ProteomeXchange.

Title	Role of ADAR1 in human macrophages
Description	This study aims to delineate the role of ADAR1 in human cells beyond its canonical function as an interferon-stimulated gene (ISG). Human monocyte-derived macrophages were subjected to RNA interference targeting either pan-ADAR1 (both isoforms) or the p150 isoform specifically. Quantitative proteomic analyses were performed using state-of-the-art isobaric tandem mass tag (TMT) labeling coupled with liquid chromatography–tandem mass spectrometry (LC-MS/MS). Strikingly, silencing of ADAR1 significantly impacted prototypical macrophage functions, including endocytosis, cholesterol metabolism, and lysosomal processing, without concurrent induction of pro-inflammatory or ISG responses. Pan-ADAR1 silencing resulted in upregulation of proteins involved in lysosomal function and cholesterol processing, whereas selective depletion of ADAR1-p150 led to downregulation of pathways associated with phagocytosis and endocytosis. Moreover, loss of pan-ADAR1 caused a marked downregulation of cell-cycle and p53 signaling pathways, suggesting a potential role for the ADAR1-p110 isoform in regulating cell proliferation. Collectively, these data define previously unappreciated proteomic alterations driven by ADAR1 that influence fundamental macrophage functions beyond its established ISG role.
HostingRepository	PRIDE
AnnounceDate	2026-02-13
AnnouncementXML	Submission_2026-02-13_01:37:23.479.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mahshid Zarrineh
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-26 12:07:07	ID requested
⏵ 1	2026-02-13 01:37:24	announced

Dataset with its publication pending

submitter keyword: Macrophages, Human,ADAR1

Prof Janne Lehtiö
contact affiliation	Karolinska Institute/ScilifeLab
contact email	janne.lehtio@ki.se
lab head
Mahshid Zarrineh
contact affiliation	Department of Oncology-pathology, Science for Life laboratory, Karolinska institute
contact email	mahshid.zarrineh@scilifelab.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD073582
     1. Label: PRIDE project
     2. Name: Role of ADAR1 in human macrophages