PXD073562 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Cell-Type–Resolved Proteomics Reveals Distinct Immune and Metabolic Programs between Primary and Recurrent Ascites in High-Grade Serous Ovarian Cancer |
| Description | This study presents a cell-type–resolved quantitative proteomics analysis of paired primary and recurrent ascites samples from patients with high-grade serous ovarian carcinoma (HGSOC). Ascites samples were collected from three patients (IROC60, IROC65, and IROC126) at diagnosis and upon disease recurrence following platinum–taxane chemotherapy. For each sample, ascites-derived cells were fractionated into unsorted, CD45⁻ (tumor-enriched), and CD45⁺ (immune-enriched) populations using magnetic-activated cell sorting. Using label-free LC–MS/MS proteomics, we quantified proteome-wide changes associated with disease recurrence across distinct cellular compartments. Pathway-level analyses revealed consistent suppression of interferon signaling across tumor and immune fractions, alongside enhanced oxidative phosphorylation and metabolic reprogramming in recurrent tumor cells. Immune-enriched fractions exhibited signatures of immunosuppression, including reduced antigen presentation and enrichment of regulatory and monocyte-related pathways. Together, this dataset provides a comprehensive proteomic resource for investigating tumor–immune interactions and recurrence-associated molecular remodeling within the ovarian cancer ascites microenvironment. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-18 |
| AnnouncementXML | Submission_2026-08-18_06:33:31.412.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | David Goodlett |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetic acid derivatized residue |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-01-26 05:49:44 | ID requested | |
| ⏵ 1 | 2026-08-18 06:33:31 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
Contact List
| David Goodlett |
|---|
| contact affiliation | University of Victoria |
| contact email | goodlett@uvic.ca |
| lab head | |
| David Goodlett |
|---|
| contact affiliation | University of Victoria |
| contact email | goodlettlabuvic@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD073562 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD073562
- Label: PRIDE project
- Name: Cell-Type–Resolved Proteomics Reveals Distinct Immune and Metabolic Programs between Primary and Recurrent Ascites in High-Grade Serous Ovarian Cancer
