PXD073550 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Inhibiting Mrt4-rRNA interaction with fumaramidmycin-based derivatives as an unexploited antifungal strategy-qualitative proteomic |
| Description | The rise of drug resistance and limitations of current antifungal treatments highlight the urgent need for innovative antifungal strategies. Here we present the development of cis-fumaramidmycin-derived analogues as a novel chemotype inhibiting the interactions of ribosome assembly factor Mrt4 with rRNA to combat fungal infections. Through antifungal screening, we identified a promising lead 20 with strong efficacy against various drug-resistant fungi, including notorious super-fungus Candida auris. A comprehensive approach combining active-and-inactive-based protein profiling (AIBPP), chemical-genetic profiling, and fluorescence polarization revealed that the antifungal activity of 20 is primarily due to selectively inhibiting essential CaMrt4-rRNA interaction by conjointly covalent engaging C96&C189 on CaMrt4 but inactive for HuMrt4-rRNA interaction, thereby disrupting fungal ribosomal assembly. Therapeutic efficacy of 20 in both Galleria mellonella larvae and murine candidiasis models validate this antifungal strategy. Collectively, our studies provide a new and much-need therapeutic strategy to address the rapidly rising burden of drug-resistant fungal infections. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-27 |
| AnnouncementXML | Submission_2026-04-26_16:10:57.793.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Hu Xiuqi |
| SpeciesList | scientific name: Candida albicans (Yeast); NCBI TaxID: NEWT:5476; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-01-26 00:43:07 | ID requested | |
| ⏵ 1 | 2026-04-26 16:10:58 | announced | |
Publication List
| 10.1038/s41467-026-70226-4; |
| Cao H, Tu J, Chen J, Chen B, Huang Z, Wang Y, Shen J, Hu X, Bo J, Rao L, Liu Z, Makunga NP, Hameed MS, Su C, Wan J, Chang W, Sheng C, Ren Y, Inhibiting Mrt4-rRNA interaction with fumaramidmycin-based derivatives as an antifungal strategy. Nat Commun, 17(1):(2026) [pubmed] |
Keyword List
| submitter keyword: ribosome assembly factor, Azole-resistant fungi, protein-RNA interaction,Fumaramidmycin-based derivatives, target identification |
Contact List
| Jian Wan |
|---|
| contact affiliation | State Key Laboratory of Green Pesticide, College of Chemistry, Central China Normal University, Wuhan 430079, China |
| contact email | jianwan@mail.ccnu.edu.cn |
| lab head | |
| Hu Xiuqi |
|---|
| contact affiliation | central china normal university |
| contact email | hxq981111@126.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD073550 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD073550
- Label: PRIDE project
- Name: Inhibiting Mrt4-rRNA interaction with fumaramidmycin-based derivatives as an unexploited antifungal strategy-qualitative proteomic
