PXD073467-1

PXD073467 is an original dataset announced via ProteomeXchange.

Title	Core-dependent sheath architecture dictates Leptospira endoflagellar curvature
Description	Spirochete bacteria include some of the most invasive human pathogens, causing diseases such as syphilis, Lyme disease, and leptospirosis that collectively affect millions of people annually. Their unique ability to penetrate tissues and cross biological barriers depends on periplasmic flagellar filaments that transmit rotational force while maintaining precise curvature to deform the cell body [1, 2]. How these filaments achieve the mechanical properties required to drive motility has remained unclear, owing to the lack of complete high-resolution structures [3, 4]. Here we present atomic structures of complete endoflagellar filaments from Leptospira species, integrating cryo-electron microscopy, mass spectrometry, and X-ray crystallography. We show that these filaments possess unprecedented complexity, with more than ten distinct proteins asymmetrically sheathing the flagellin core. The isoform identity of the core flagellin is coupled to sheath organization, together dictating filament curvature. This architectural plasticity is critical for motility in viscous environments and during infection. The conservation of several core and sheath proteins across the phylum suggests that Spirochetes exploit modular core-sheath coupling to optimize motility across environments of varying viscosity, a capability critical for both environmental adaptation and tissue invasion by pathogens.
HostingRepository	PRIDE
AnnounceDate	2026-07-14
AnnouncementXML	Submission_2026-07-14_11:44:48.029.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martial Rey
SpeciesList	scientific name: Leptospira biflexa; NCBI TaxID: NEWT:172;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-23 11:10:43	ID requested
⏵ 1	2026-07-14 11:44:48	announced

Dataset with its publication pending

submitter keyword: Structural Biology, XL-MS,Leptospira endoflagellum

Julia Chamot-Rooke
contact affiliation	Institut Pasteur, Université Paris Cité, CNRS UAR 2024, Mass Spectrometry for Biology Unit, Paris, France
contact email	julia.chamot-rooke@pasteur.fr
lab head
Martial Rey
contact affiliation	CNRS, Pasteur
contact email	martial.rey@pasteur.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD073467

PRIDE project URI

• PRIDE
  1. PXD073467
     1. Label: PRIDE project
     2. Name: Core-dependent sheath architecture dictates Leptospira endoflagellar curvature