PXD073452-1

PXD073452 is an original dataset announced via ProteomeXchange.

Title	Identification of phosphorylations sites in Arabidopsis thaliana BSU1 recombinantly expressed in insect cells, and when incubated with the GSK3 kinase BIN2.
Description	Brassinosteroids (BRs) are plant steroid hormones sensed at the cell surface by the membrane receptor kinase BRI1. Activation of BRI1 leads to the dephosphorylation of BZR1/BES1 transcription factors through inactivation of the glycogen synthase kinase 3 BIN2. Here, the in vitro phosphorylation sites of the isolated BSU1 Kelch domain (residues 11-351), phosphatase domain (residues 421-793) and of full-length BSU1 (residues 1-793) recombinantly expressed in baculovirus-infected Spodoptera frugiperda cells were identified in isolation (labelled ctrl) or after incubation with the GSK3 kinase BIN2, expressed as a thioredoxin A fusion protein in E. coli. Reactions were performed in the presence of 100 micromolar ATP and 1 mM MgCl2 for 1:30 h at room temperature.
HostingRepository	PRIDE
AnnounceDate	2026-05-22
AnnouncementXML	Submission_2026-05-22_09:20:52.667.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD073452
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Michael Hothorn
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-23 06:45:46	ID requested
⏵ 1	2026-05-22 09:20:53	announced

10.1073/pnas.2600591123;

10.6019/PXD073452;

Rico-Resendiz F, Pri-Tal O, Raia P, Moretti A, Chen H, Yu J, Broger L, Fuchs C, Hothorn LA, Loub, é, ry S, Hothorn M, Plant Kelch phosphatases are Ser/Thr phosphatases involved in cell cycle regulation. Proc Natl Acad Sci U S A, 123(21):e2600591123(2026) [pubmed]

submitter keyword: None

Michael Hothorn
contact affiliation	Structural Plant Biology Laboratory, Department of Plant Sciences, University of Geneva, 1211 Geneva, Switzerland
contact email	michael.hothorn@unige.ch
lab head
Michael Hothorn
contact affiliation	Department of Plant Science, University of Geneva
contact email	michael.hothorn@unige.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073452

PRIDE project URI

• PRIDE
  1. PXD073452
     1. Label: PRIDE project
     2. Name: Identification of phosphorylations sites in Arabidopsis thaliana BSU1 recombinantly expressed in insect cells, and when incubated with the GSK3 kinase BIN2.