PXD073419 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | ZNFX1 activity-based probe crosslinking mass spectrometry |
| Description | The aim of this experiment was to identify the catalytic cysteine in the antiviral E3 ligase ZNFX1 using activity-based probe (ABP)–enabled cross-linking mass spectrometry (XL-MS). A biotinylated E2~ubiquitin activity-based probe containing a cysteine-electrophile trap was used to covalently capture ubiquitin a transfer intermediate between UBE2D2~Ub and full-length human ZNFX1. Cross-linked peptide mapping was performed to identify probe-labelled residues and putative catalytic site. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-05-22 |
| AnnouncementXML | Submission_2026-05-22_10:50:28.405.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Satpal Virdee |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-01-22 11:52:08 | ID requested | |
| ⏵ 1 | 2026-05-22 10:50:29 | announced | |
Publication List
| Squair DR, Rivers E, Sowar H, Balci A, Harmo R, Wright DJ, Beniwal G, Soetens M, Mathur S, Tollervey A, Wood NT, Pao KC, Stanton C, Fletcher AJ, Virdee S, ZNFX1 uses two-component ubiquitin circuitry to quarantine viral RNA. Mol Cell, 86(6):1164-1181.e12(2026) [pubmed] |
| 10.1016/j.molcel.2026.02.015; |
Keyword List
Contact List
| Satpal Virdee |
|---|
| contact affiliation | MRC Protein Phosphorylation and Ubiquitylation Unit, University of Dundee, UK |
| contact email | s.s.virdee@dundee.ac.uk |
| lab head | |
| Satpal Virdee |
|---|
| contact affiliation | University of Dundee |
| contact email | s.s.virdee@dundee.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD073419
- Label: PRIDE project
- Name: ZNFX1 activity-based probe crosslinking mass spectrometry
