PXD073407-1

PXD073407 is an original dataset announced via ProteomeXchange.

Title	Cross-species multi-omic analyses of great ape ejaculates reveal novel strategies for enhancing sperm cryopreservation in Bornean orangutan (Pongo pygmaeus)
Description	The orangutan (Pongo spp., Linnaeus, 1760) is the most endangered great ape, classified as critically endangered. Sperm cryopreservation is a valuable tool for banking genetic resources and solving the complexities of relocating animals; however, cryopreservation protocols remain suboptimal for this species. Due to their phylogenetic closeness, this study aimed to explore the applicability of a chimpanzee (Pan troglodytes, Blumenbach, 1775) sperm cryopreservation protocol to orangutans. To guide further modifications to the protocol, we revealed, for the first time, the comprehensive lipidomic and proteomic characterizations of orangutan ejaculates with parallel comparisons to chimpanzee ejaculates. Functional analyses of oxidative and osmotic stress responses were also conducted to provide valuable evidence of the physiological changes and defense mechanisms associated with sperm cryodamage. The cross-species multi-omic analyses showed that compared to chimpanzee, the orangutan sperm lipid profile presented significant alterations after the freezing-thawing process, notably showing significant cholesterol loss. While interspecies differences in antioxidant enzyme composition and activity were observed, insufficient evidence supported the orangutan's heightened susceptibility to oxidative stress. Conversely, orangutan sperm exhibited low tolerance to hypoosmotic conditions. To prevent cryodamage, a modified thawing protocol by implementing a serial dilution approach significantly minimized the hypoosmotic shock and improved post-thaw motility to 19%. In conclusion, this study presents the first complete proteome and lipidome analyses of chimpanzee and orangutan ejaculates, providing valuable insights into the physiological changes and defense mechanisms associated with sperm cryopreservation. This knowledge enabled a science-based approach to improving cryopreservation protocols, moving away from trial-and-error methods.
HostingRepository	PRIDE
AnnounceDate	2026-03-08
AnnouncementXML	Submission_2026-03-08_10:59:10.191.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laura Orama Méar
SpeciesList	scientific name: Pongo pygmaeus (Bornean orangutan); NCBI TaxID: NEWT:9600; scientific name: Pan troglodytes (Chimpanzee); NCBI TaxID: NEWT:9598;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-22 06:15:11	ID requested
⏵ 1	2026-03-08 10:59:10	announced

10.1186/S40851-026-00262-X;

submitter keyword: chimpanzee,orangutan, seminal plasma, sperm, ejaculate

Pei-Shiue Tsai
contact affiliation	Graduate Institute of Veterinary Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., 10617, Taipei, Taiwan
contact email	psjasontsai@ntu.edu.tw
lab head
Laura Orama Méar
contact affiliation	Leibniz Institute of Zoo and Wildlife research
contact email	mearlauraorama@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD073407
     1. Label: PRIDE project
     2. Name: Cross-species multi-omic analyses of great ape ejaculates reveal novel strategies for enhancing sperm cryopreservation in Bornean orangutan (Pongo pygmaeus)