PXD073401-1

PXD073401 is an original dataset announced via ProteomeXchange.

Title	Mapping Myc Interactome by Micro-Affinity Purification Coupling Mass Spectrometry
Description	Affinity purification-mass spectrometry (AP-MS) is a powerful approach for protein-protein interaction (PPI) analysis. Here, we report the development of a micro-scale immobilized metal affinity chromatography (m-IMAC) platform for rapid and reproducible purification of protein complexes prior to mass spectrometric analysis. The method was applied for mapping PPIs of Myc protein which regulates the expression of thousands of genes and plays a central role in tumorigenesis. Systematic characterization of Myc interactome is essential for understanding how a single transcription factor orchestrates such diverse biological processes. Affinity purification was performed using m-IMAC columns fabricated by photoinitiated polymerization within pipette tips (spin-tip columns), enabling efficient enrichment of Myc interacting proteins. Analysis with Label-Free Quantification (LFQ) with Data-Independent Acquisition (DIA) proteomics identified 574 Myc interacting proteins, the majority of which have not been previously reported as the Myc interactors. Two newly identified Myc associated factors were validated by biochemical assays, and the overall dataset showed good concordance with the reported Myc interactors. Gene Ontology (GO) enrichment analysis of the Myc associated proteome revealed significant enrichment in macromolecular metabolism (particularly RNA processing), regulation of gene expression, chromatin organization and remodeling. This work provides new insights into Myc mediated transcriptional regulation in proteomic level. Importantly, owing to its straightforward, rapid operation, the m-IMAC platform provides a general and efficient analytical strategy for high-throughput interactome analysis.
HostingRepository	PRIDE
AnnounceDate	2026-06-09
AnnouncementXML	Submission_2026-06-09_06:22:40.930.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ying zhu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-22 02:37:18	ID requested
⏵ 1	2026-06-09 06:22:41	announced

10.1021/ACS.JPROTEOME.6C00156;

submitter keyword: transcriptional regulation mechanisms, Proteomics, Affinity Purification-Mass Spectrometry, c-Myc

Jingwu Kang
contact affiliation	State Key Laboratory of Chemical Biology, Center for Excellence in Molecular Synthesis, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences
contact email	jingwu.kang@sioc.ac.cn
lab head
ying zhu
contact affiliation	School of Pharmacy, East China University of Science and Technology
contact email	zhuying3@sioc.ac.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD073401
     1. Label: PRIDE project
     2. Name: Mapping Myc Interactome by Micro-Affinity Purification Coupling Mass Spectrometry