PXD073289-1

PXD073289 is an original dataset announced via ProteomeXchange.

Title	Enrichment of HLA-DR+ neutrophils in osteoarthritic infrapatellar fat pad
Description	Neutrophils are increasingly recognized as functionally versatile immune cells, with tissue-specific phenotypes that extend beyond their classical role in acute inflammation. In osteoarthritis (OA), neutrophils are abundant in synovial fluid (SF) which has been linked to worse symptoms., yet their presence and function within the infrapatellar fat pad (IFP) remain poorly defined. Here, we characterized neutrophils in OA IFP using flow cytometry, immunofluorescence, proteomics, and functional assays. Weand identified a distinct population of HLA-DR⁺ neutrophils enriched in OA IFP. compared to matched SF. These cells co-expressed classical neutrophil markers and antigen presentation markers. Sorted IFP HLA-DR+ neutrophils induced proliferation of autologous CD3⁺ T cells, supporting a role in antigen presentation. Proteomic profiling of OA IFP revealed upregulation of metabolic and enzymatic activities as well as downregulation of apoptotic and enzyme inhibition pathways, consistent with the increased fibrosis and neovascularization observed histologically. Our findings suggest that IFP-resident neutrophils adopt a tissue adapted, antigen-presenting phenotype, with potential roles in T cell activation, fibrosis, and angiogenesis. Collectively, our findings expand the functional scope of neutrophils in arthritic diseases, further establishing their potential in antigen presentation, and positions the IFP as a previously underappreciated, immunologically active niche in OA pathogenesis.
HostingRepository	PRIDE
AnnounceDate	2026-03-23
AnnouncementXML	Submission_2026-03-22_17:37:22.106.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Susana Bravo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-20 09:34:19	ID requested
⏵ 1	2026-03-22 17:37:23	announced

10.1016/j.isci.2026.115214;

Bevc K, Zhang S, Pazos A, Berest I, Fonti M, Salzmann G, Bruhin V, Hax J, Perez AA, Gomez R, Mair F, Arnold IC, Wong MZ, neutrophils in osteoarthritic infrapatellar fat pad. iScience, 29(4):115214(2026) [pubmed]

submitter keyword: osteoarthritis,infrapatellar fat pad, neutrophils, antigen presentation

Susana B Bravo
contact affiliation	Instituto de Investigaciones Sanitarias de Santiago de Compostela
contact email	sbbravo@gmail.com
lab head
Susana Bravo
contact affiliation	FIDIS
contact email	sbbravo@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD073289
     1. Label: PRIDE project
     2. Name: Enrichment of HLA-DR+ neutrophils in osteoarthritic infrapatellar fat pad