PXD073280 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Scaffolding of the H4K5ac chromatin remodeling complex by lncRNA MAHAC mediates epithelial-mesenchymal transition |
| Description | Anchoring of a chromatin remodeler complex by long non-coding RNAs (lncRNAs) is a frequently utilized mechanism for lncRNAs to regulate gene expression. Hypoxia is a microenvironemntal condition that plays a crucial role in promoting tumor progression. We previously identified a hypoxia-inducible lncRNA, RP11-390F4.3, that regulates epithelial–mesenchymal transition (EMT) without a delineated mechanism. Here we show that the lncRNA RP11-390F4.3 (renamed MAHAC: MAintenance of Histone ACetylation) specifically induces histone H4 lysine 5 acetylation (H4K5ac) mark and promotes the enrichment of H4K5ac mark on the promoters of EMT transcription factors. MAHAC scaffolds the ILF3/NF90–ILF2–CBP complex, which is co-localized with the members of the complex inside nucleus under hypoxia. The minimal MAHAC region (nt 686–741) required for scaffolding the complex was mapped and it induces allosteric activation of H4K5ac in in vitro histone acetyltransferase assay. This minimal MAHAC region is essential for hypoxia-induced EMT, migration, invasion, and H4K5ac activation. These findings demonstrate that hypoxia-induced MAHAC represents an unexplored allosteric regulator of H4K5ac that activates EMT and induces tumor progression. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-16 |
| AnnouncementXML | Submission_2026-02-15_16:26:46.649.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD073280 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Pei-Hua Peng |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | LTQ Orbitrap Elite |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-01-20 06:52:08 | ID requested | |
| ⏵ 1 | 2026-02-15 16:26:48 | announced | |
Publication List
| Hsu KW, Chang JS, Lai JC, Yang LH, Peng PH, Lin TC, Wu KJ, Scaffolding of the H4K5ac chromatin remodeling complex by lncRNA MAHAC mediates epithelial-mesenchymal transition. Nucleic Acids Res, 54(4):(2026) [pubmed] |
| 10.1093/nar/gkag101; |
| 10.6019/PXD073280; |
Keyword List
| submitter keyword: MAHAC,lncRNA, ILF3/NF90–ILF2–CBP complex, H4K5ac, tumor progression |
Contact List
| Kai-Wen, Hsu |
|---|
| contact affiliation | Institute of Translational Medicine and New Drug Development, Research Center for Cancer Biology, China Medical University |
| contact email | kwhsu@mail.cmu.edu.tw |
| lab head | |
| Pei-Hua Peng |
|---|
| contact affiliation | Institute of Translational Medicine and New Drug Development, Research Center for Cancer Biology, China Medical University |
| contact email | jinger0908@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD073280
- Label: PRIDE project
- Name: Scaffolding of the H4K5ac chromatin remodeling complex by lncRNA MAHAC mediates epithelial-mesenchymal transition
