PXD073269-1

PXD073269 is an original dataset announced via ProteomeXchange.

Title	A proteomic signature of oocyte quality from models of varying oocyte developmental competence
Description	Within the ovarian follicle, mammalian oocytes acquire the necessary molecular machinery for successful fertilisation and embryonic development. In the absence of the follicle, oocytes matured in vitro (IVM) using traditional IVM show compromised developmental competence, resulting in poorer outcomes for patients. A novel in vitro system (CAPA-IVM) using c-type natriuretic peptide provides the oocyte with extended time to develop, with improved success rates. Here, we interrogate constituents of oocyte developmental competence by comparing the proteomic signature of mature mouse metaphase II (MII) cumulus-oocyte complexes (COCs) of differing intrinsic qualities, as matured by various maturation protocols. We employed label-free mass spectrometry to analyse the proteomes of mouse oocytes and cumulus cells separately. Proteomic profiling identified around 1600 proteins in oocytes and 3100 in cumulus cells across all three treatment groups (at least 2 peptides per protein). Differential expression analysis and pattern analysis collectively revealed a signature of proteins that were consistently differentially expressed between in vivo and in vitro oocyte maturation systems (log2FC of ± 1 and a p-value ≤ 0.05). These subsets of proteins were mapped to biological processes including eukaryotic translation, autophagy and endocytosis pathways within oocytes. Changes in reactive oxygen species detoxification and serine biosynthesis were observed in cumulus cells. Altogether, these findings provide new insights into the proteomic mechanisms underpinning oocyte developmental competence, with the potential to improve clinical oocyte diagnostics, expand on current clinical maturation systems and provide novel therapeutic options to infertile patients.
HostingRepository	PRIDE
AnnounceDate	2026-08-24
AnnouncementXML	Submission_2026-08-23_16:58:12.442.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD073269
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Emily Frost
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-19 21:30:37	ID requested
⏵ 1	2026-08-23 16:58:13	announced

10.1093/humrep/deag134;

10.6019/PXD073269;

Frost ER, Richani D, Poljak A, Vuyyuru A, Liao X, Georgiou E, Gunasekara JMB, Mihalas BP, Sucquart IE, Kadam K, Wu LE, Gilchrist RB, A proteomic signature of oocyte quality from models of varying oocyte developmental competence. Hum Reprod, ():(2026) [pubmed]

submitter keyword: Capacitation-IVM (CAPA-IVM), assisted reproductive technologies, folliculogenesis,Oocyte, cumulus cell, in vitro maturation (IVM)

Robert Bruce Gilchrist
contact affiliation	Fertility & Research Centre, School of Clinical Medicine, Faculty of Medicine & Health, University of New South Wales, Australia
contact email	r.gilchrist@unsw.edu.au
lab head
Emily Frost
contact affiliation	University of New South Wales
contact email	emily.frost@unsw.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD073269
     1. Label: PRIDE project
     2. Name: A proteomic signature of oocyte quality from models of varying oocyte developmental competence