PXD073251-1

PXD073251 is an original dataset announced via ProteomeXchange.

Title	LC-MS analysis of human melanoma cell line response to eIF4F inhibition and MEK inhibition
Description	The project aimed to identify changes at the protein level in human melanoma cells treated with the eIF4F inhibitor rocaglamide A, the MEK inhibitor PD184352 (CI-1040), and their combination. A375 and MelJuso human melanoma cell lines were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum, 1% penicillin/streptomycin, and 2 mM L-glutamine and maintained at 37 °C in a humidified atmosphere containing 5% CO2. For the analysis, both cell lines were seeded in 10-mm dishes and, the following day, treated with 100 nM rocaglamide A (DRG), 500 nM PD184352/CI-1040 (MEKi), and their combination (DRG_MEKi). Negative controls (CTR) were treated with an equivalent volume of DMSO. 20 h post-treatment, cells were washed with PBS, collected, and pelleted.
HostingRepository	PRIDE
AnnounceDate	2026-06-15
AnnouncementXML	Submission_2026-06-14_18:04:20.217.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Potesil
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-19 14:07:27	ID requested
⏵ 1	2026-06-14 18:04:21	announced

Vadovi, č, ov, á N, Le, š, kov, á A, Ko, ž, do, ň, ov, á K, Smolkov, á K, Val, č, í, kov, á B, Kafka F, Pot, ě, š, il D, Zdr, á, hal Z, Vacek O, V, í, chov, á R, Sou, č, ek K, Uldrijan S, Translational control of AMPK activity in melanoma. Cell Commun Signal, 24(1):(2026) [pubmed]

10.1186/s12964-026-02901-4;

submitter keyword: eIF4A, human, inhibitor, rocaglamide A, small-molecule, CI-1040,melanoma, PD184352, cell line, MelJuso, A375, LC-MS, eIF4F, MEK

Zbynek Zdrahal
contact affiliation	Proteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic
contact email	zdrahal@sci.muni.cz
lab head
David Potesil
contact affiliation	Proteomics Core Facility and Research Group Proteomics CEITEC-MU Masaryk University Brno, Czech Republic
contact email	david.potesil@ceitec.muni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD073251

PRIDE project URI

• PRIDE
  1. PXD073251
     1. Label: PRIDE project
     2. Name: LC-MS analysis of human melanoma cell line response to eIF4F inhibition and MEK inhibition