PXD073201-1

PXD073201 is an original dataset announced via ProteomeXchange.

Title	Systematic cysteine scanning identifies a druggable pocket in oncogenic KRAS
Description	We developed Cysteine Mapping of Accessible Pockets (CysMAP), a method for identifying druggable pockets in proteins. CysMAP employs systematic pooled cysteine variant libraries screened against diverse covalent compound libraries by intact LC-MS. Here, we applied CysMAP to 189 KRAS(G12D) variants, purifying KRAS Cys-variants and screening them against 47 covalent compounds, quantifying accessibility and reactivity across KRAS(G12D).
HostingRepository	PRIDE
AnnounceDate	2026-02-06
AnnouncementXML	Submission_2026-02-05_18:53:50.840.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	L. Moore van Tienen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	protein modification
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-18 07:28:18	ID requested
⏵ 1	2026-02-05 18:53:51	announced

10.1016/J.CHEMBIOL.2026.01.007;

submitter keyword: None

William R
contact affiliation	Broad Institute
contact email	thesellerslab@broadinstitute.org
lab head
L. Moore van Tienen
contact affiliation	Broad Institute
contact email	lmvantienen@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD073201

PRIDE project URI

• PRIDE
  1. PXD073201
     1. Label: PRIDE project
     2. Name: Systematic cysteine scanning identifies a druggable pocket in oncogenic KRAS