PXD073161-1

PXD073161 is an original dataset announced via ProteomeXchange.

Title	AB138 integrates prophylactic, therapeutic, and immunomodulatory functions in cells
Description	To elucidate the antiviral mechanism of AB138 in cells, we performed quantitative proteomic analysis on HCT-8 cells under three conditions: AB138 treatment alone, pre-treatment with 1 μM AB138 for 8 h prior to BCoV infection, and treatment with 1 μM AB138 initiated 24 h after BCoV infection. Consistent with host-target engagement, volcano plot analyses revealed extensive yet selective remodeling of the proteome in response to AB138 treatment across dosing strategies (Fig. 4G). In cells treated with AB138 alone, GSPT1 was the most substantially reduced protein, although IKZF1/3 were not detected. Following post-infection administration of AB138, significant decreases were observed in GSPT1, pro-inflammatory factors (e.g., NFKBIA, IL17RA), and apoptosis-regulating proteins (e.g., BAD, BCL2L2), whereas the interferon-associated protein IFRD1 was upregulated. In contrast, in cells pretreated with AB138 prior to BCoV infection, substantial reductions were observed in GSPT1 as well as immune-related proteins (e.g., NFKBIA, IL6ST). Conversely, levels of BAD and IFRD1 were markedly increased. These results indicate that AB138 exerts its antiviral effects, at least in part, through modulation of host inflammatory and apoptotic pathways in cells.
HostingRepository	iProX
AnnounceDate	2026-01-16
AnnouncementXML	Submission_2026-01-16_00:04:10.468.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luo Xing
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-16 00:03:55	ID requested
⏵ 1	2026-01-16 00:04:11	announced

Dataset with its publication pending

submitter keyword: label-free, DIA, Astral, Vanquish Neo, HCT-8, BCoV, AB138, pretreatment, post-infection

Bin Zhang
contact affiliation	Southwest Minzu University
contact email	binovy@sina.com
lab head
Luo Xing
contact affiliation	Southwest Minzu University
contact email	luoxing@genepre.com
dataset submitter

iProX dataset URI