⮝ Full datasets listing
PXD073138-2
PXD073138 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Phosphorylation of WDR48 by phototropins drives starch degradation to promote stomatal opening |
| Description | Plant stomata open in response to blue light under a background of red light. Red light induces starch accumulation through guard cell photosynthesis, whereas blue light causes rapid starch degradation via the activation of plasma membrane H+-ATPase through phosphorylation of BLUS1 by phototropins, thus promoting stomatal opening. However, how phototropins mediate starch degradation remains unclear. In this study, we identified WD-repeat protein 48 (WDR48), which is essential for starch degradation. Phosphoproteome analysis revealed that WDR48 was phosphorylated in response to blue light. Mutation of WDR48 impaired starch degradation and stomatal opening by blue light. Phototropins interacted with and phosphorylated WDR48. WDR48 and BLUS1 constituted separate signalling pathways required for starch degradation. We propose that the coordinated control of starch synthesis by red light and its degradation by blue light signalling through WDR48 and BLUS1 is a key mechanism for stomatal opening by red and blue light. |
| HostingRepository | jPOST |
| AnnounceDate | 2026-02-18 |
| AnnouncementXML | Submission_2026-03-23_17:52:31.313.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Naoyuki Sugiyama |
| SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
| ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-01-15 20:11:44 | ID requested | |
| 1 | 2026-02-17 16:45:59 | announced | |
| ⏵ 2 | 2026-03-23 17:52:32 | announced | 2026-03-24: Updated PubMed. |
Publication List
| Yamauchi S, Fuji S, Ikuta H, Sugiyama N, Kodama Y, Distefano L, Fujii H, Yamashita K, Takase H, Nomoto M, Tada Y, Umezawa T, Hotta K, Santelia D, Shimazaki KI, Takemiya A, Phosphorylation of WDR48 by phototropins drives starch degradation to promote stomatal opening. Nat Commun, ():(2026) [pubmed] |
Keyword List
| submitter keyword: Arabidopsis, phosphoproteome |
Contact List
| Atsushi Takemiya | |
|---|---|
| lab head | |
| Naoyuki Sugiyama | |
| contact affiliation | Kyoto University |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST004327/ |
