PXD073093-1

PXD073093 is an original dataset announced via ProteomeXchange.

Title	Paternal dietary macronutrients affect the seminal vesicle fluid proteome and foetal development
Description	Seminal fluid can have wide-ranging effects on reproductive fitness – from affecting sperm fertilisation capacity and female reproductive physiology, to influencing offspring viability and health. Seminal fluid can also change in response to environmental conditions including diet, which of itself is known to affect reproductive traits and fitness outcomes. However, an understanding of how paternal diet alters seminal fluid composition and how these effects relate to foetal development remains elusive. Here, we applied the Geometric Framework for Nutrition to systematically manipulate dietary macronutrient balance in male mice and determine dietary effects on the seminal vesicle fluid (SVF) proteome and related differences to aspects of foetal development in offspring sired by these males. We 1) identify and determine the largest number of proteins in the mouse SVF proteome to date, 2) identify a set of proteins that are significantly affected by dietary macronutrient balance, 3) show that differences in a protein related to lipid mobilisation and metabolism (APOA4) are correlated with foetal development, and 4) detect dietary effects on aspects of foetal development that were unrelated to SVF protein abundance. This study provides a comprehensive characterisation of the male SVF proteome across nutritional space and highlights potential functional ways in which male diet and the seminal fluid may mediate fitness.
HostingRepository	PRIDE
AnnounceDate	2026-07-23
AnnouncementXML	Submission_2026-07-22_22:04:58.591.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lewin Small
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-14 20:04:51	ID requested
⏵ 1	2026-07-22 22:04:59	announced

Macartney EL, Senior AM, Small L, Crean AJ, Pini T, Pulpitel TJ, Nobrega MA, Barr, è, s R, Simpson SJ, Paternal dietary macronutrients affect the seminal vesicle fluid proteome and fetal development: a geometric framework for nutrition study in mice. Proc Biol Sci, 293(2073):(2026) [pubmed]

10.1098/rspb.2026.0535;

submitter keyword: None

Lewin Small
contact affiliation	Charles Perkins Centre, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW, 2006, Australia.
contact email	lewin.small@sydney.edu.au
lab head
Lewin Small
contact affiliation	University of Sydney
contact email	lewin.small@sydney.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD073093

PRIDE project URI

• PRIDE
  1. PXD073093
     1. Label: PRIDE project
     2. Name: Paternal dietary macronutrients affect the seminal vesicle fluid proteome and foetal development