PXD073084-1

PXD073084 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis of Addiction-Related Brain Regions in Mice Following Conditioned Place Preference Training with 2-Fluoroketamine and Ketamine；Proteomic Profiling of Primary Mouse Neurons Following Exposure to 2-Fluoroketamine and Ketamine
Description	To evaluate the addictive potential of 2-FDCK (20mg/kg) and KET (20mg/kg) in mice, we employed a standard conditioned place preference (CPP) paradigm. During the conditioning phase, mice learned to associate the abused drug reward with a specific contextual chamber. After a 21-day conditioning period, the mice were reintroduced to the CPP apparatus and allowed to freely explore both chambers in the absence of the drug. Subsequently, samples from the hippocampus, mPFC, and VTA were collected and pretreated. We also extracted primary neurons from the mouse prefrontal cortex as an in vitro model, exposed them to 2-FDCK or KET (30uM), and collected the samples. All samples were then analyzed using an Eksigent® M5+TripleTOF® 5600+ system. All data acquisition was performed using Analyst TF 1.7 software, and the IDA data were searched against the database using ProteinPilot™ software.
HostingRepository	iProX
AnnounceDate	2026-01-14
AnnouncementXML	Submission_2026-02-11_00:03:10.320.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiaying Wang
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-14 18:31:30	ID requested
⏵ 1	2026-02-11 00:03:10	announced

Dataset with its publication pending

submitter keyword: 2-Fluoroketamine, Ketamine, Addiction, Brain, Primary Neurons

Yuan Xie
contact affiliation	China Pharmaceutical University
contact email	yuanxie@cpu.edu.cn
lab head
Jiaying Wang
contact affiliation	China Pharmaceutical University
contact email	13022512223@163.com
dataset submitter

iProX dataset URI