PXD073074-1

PXD073074 is an original dataset announced via ProteomeXchange.

Title	Semaglutide targets mitochondria to regulate metabolism and inflammation in sarcopenia and osteoarthritis
Description	Metabolic and inflammatory diseases often coexist, and become important challenges facing global health. As a traditional anti-obesity drug, semaglutide is reported to have therapeutic effects in sarcopenia and osteoarthritis (OA), but its mechanism of action still needs to be explored. Here we employed single-cell sequencing analysis to analyze transcriptome changes cross various tissues of high-fat diet (HFD) and OA model mice, and found that semaglutide significantly improved the metabolic and inflammatory disorders of muscle tissue during HFD and OA. Mechanistically, we found that semaglutide could enhance muscle mitochondrial metabolism and mitophagy, alleviated the activation of inflammatory cytokines caused by hypoxia, and regulated the progression of sarcopenia and OA across tissues. Intramuscular injection of mitochondria, pre-stimulated by semaglutide, could significantly alleviate OA inflammation and pain symptoms. These findings reveal the mitochondrial regulatory mechanism in the muscle-OA axis and provide a new perspective for the application of semaglutide in OA treatment.
HostingRepository	PRIDE
AnnounceDate	2026-05-06
AnnouncementXML	Submission_2026-05-06_06:07:10.326.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	yuchen tian
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral Zoom

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-14 12:58:20	ID requested
⏵ 1	2026-05-06 06:07:10	announced

Dataset with its publication pending

submitter keyword: c2c12, semaglutide,mitochondria

Tian Yuchen
contact affiliation	Shanghai Sixth People's Hospital, Shanghai
contact email	yctian97s@sjtu.edu.cn
lab head
yuchen tian
contact affiliation	Shanghai Sixth People's Hospital
contact email	yctian97s@sjtu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073074

PRIDE project URI

• PRIDE
  1. PXD073074
     1. Label: PRIDE project
     2. Name: Semaglutide targets mitochondria to regulate metabolism and inflammation in sarcopenia and osteoarthritis