PXD073058 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Mechanism of beta-arrestin 1 mediated Src activation via Src SH3 domain revealed by cryo-electron microscopy |
| Description | Beta-arrestins (βarrs) are key regulators and transducers of G-protein coupled receptor signaling; however, little is known of how βarrs communicate with their downstream effectors. Here, we report the first structural insights into the fundamental mechanisms driving βarr-mediated signal transduction. Using cryo-electron microscopy, we elucidate how βarr1 recruits and activates the non-receptor tyrosine kinase Src, the first identified signaling partner of βarrs. βarr1 engages Src SH3 through two distinct sites, each employing a different recognition mechanism: a polyproline motif in the N-domain and a non-proline-based interaction in the central crest region. At both sites βarr1 interacts with the aromatic surface of SH3, disrupting the autoinhibited conformation of Src and directly triggering its allosteric activation. This structural evidence establishes βarr1 as an active regulatory protein rather than a passive scaffold and suggests a potentially general mechanism for βarr-mediated signaling across diverse effectors. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-01-27 |
| AnnouncementXML | Submission_2026-01-26_19:16:12.185.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Kunhong Xiao |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro 2 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-01-14 07:21:35 | ID requested | |
| ⏵ 1 | 2026-01-26 19:16:13 | announced | |
Publication List
| 10.1101/2024.07.31.605623; |
| Pakharukova N, Thomas BN, Bansia H, Li L, Bassford DK, Abzalimov RR, Kim J, Kahsai AW, Pani B, Xiao K, Ochakovski R, Liu S, Zhang X, Ahn S, des Georges A, Lefkowitz RJ, Mechanism of beta-arrestin 1 mediated Src activation via Src SH3 domain revealed by cryo-electron microscopy. bioRxiv, ():(2025) [pubmed] |
Keyword List
| submitter keyword: Src activation,beta-arrestin, CXMS, Src, LC-MS/MS |
Contact List
| Kunhong Xiao |
|---|
| contact affiliation | Center for Proteomics & Artificial Intelligence, Allegheny Health Network Cancer Institute, Pittsburgh, PA 15202, USA |
| contact email | kevin.kh.xiao@gmail.com |
| lab head | |
| Kunhong Xiao |
|---|
| contact affiliation | AHN |
| contact email | kevin.kh.xiao@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD073058
- Label: PRIDE project
- Name: Mechanism of beta-arrestin 1 mediated Src activation via Src SH3 domain revealed by cryo-electron microscopy
