PXD072931-1

PXD072931 is an original dataset announced via ProteomeXchange.

Title	Characterization of an animal polyhydroxyalkanoate depolymerase that degrades microbial PHAs
Description	Microbial species produce the storage biopolymer polyhydroxyalkanoate (sPHA), as a carbon and energy source. However, only bacteria and fungi are currently known to degrade microbial sPHAs, using an enzyme called PHA depolymerase (PHAD). This study shows that animals also have a PHAD that can degrade sPHA. We discovered a PHAD in the gutless oligochaete, Olavius algarvensis. These marine worms gain their nutrition by digesting their bacterial symbionts, with sPHA making up to 42% of the carbon in their dominant symbiont. In gutless oligochaetes, this sPHA-synthesizing symbiont is present in the post-genital trunk regions and absent in the head region of the worm (Ott et al., Springer 2023). Based on this previous observation, the worms were dissected to remove the head portion (“tip”) from the body (“trunk”) just above the genital pad region. Samples consisted of single worms and pools of three or five worms washed in 99% ethanol prior to dissection (n = 3 for single and pooled samples). In addition, one sample set consisted of single worms not washed with ethanol (n = 3). Dissected samples were placed in 2 ml cryo-vials, flash frozen in liquid nitrogen, and stored at -80 °C prior to protein digestion. The whole sample set was processed for proteomics.
HostingRepository	PRIDE
AnnounceDate	2026-07-28
AnnouncementXML	Submission_2026-07-28_03:11:22.842.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Grace D'Angelo
SpeciesList	scientific name: Olavius algarvensis Delta 1 endosymbiont; NCBI TaxID: NEWT:334747; scientific name: Olavius algarvensis spirochete endosymbiont; NCBI TaxID: NEWT:260710; scientific name: Candidatus Thiosymbion; NCBI TaxID: NEWT:1907535; scientific name: Olavius algarvensis; NCBI TaxID: NEWT:188229; scientific name: Olavius algarvensis Gamma 3 endosymbiont; NCBI TaxID: NEWT:260704; scientific name: Olavius algarvensis Gamma 1 endosymbiont; NCBI TaxID: NEWT:260705; scientific name: Olavius algarvensis Delta 4 endosymbiont; NCBI TaxID: NEWT:260707;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-11 23:37:08	ID requested
⏵ 1	2026-07-28 03:11:23	announced

10.1038/S41559-026-03153-8;

submitter keyword: None

Manuel Liebeke
contact affiliation	Institute of Human Nutrition and Food Science, Department of Metabolomics, University of Kiel, Germany Max Planck Institute for Marine Microbiology, Bremen, Germany (lab head)
contact email	mliebeke@metabolomics.uni-kiel.de
lab head
Grace D'Angelo
contact affiliation	Max Planck Institute for Marine Microbiology
contact email	gdangelo@mpi-bremen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD072931

PRIDE project URI

• PRIDE
  1. PXD072931
     1. Label: PRIDE project
     2. Name: Characterization of an animal polyhydroxyalkanoate depolymerase that degrades microbial PHAs