PXD072903-1

PXD072903 is an original dataset announced via ProteomeXchange.

Title	Integrated Membrane Proteomics and Machine Learning Identify HBV-Associated Membrane Proteins as Prognostic Biomarkers for Hepatocellular Carcinoma
Description	In this study, we employed two complementary proteomic strategies to identify HBV-associated membrane proteins: (1) classical membrane fractionation coupled with mass spectrometry, and (2) BS2-mediated proximity labeling-utilizing a membrane-targeted esterase from Bacillus subtilis (BS2) to enrich membrane-proximal proteins-followed by mass spectrometry. Both approaches were applied to HBV-negative and HBV-positive tumor cell lines (HepG2 and Hep3B). Subsequently, by integrating HBV‑HCC proteomic data, we identified 98 HBV‑associated membrane‑surface proteins. Through Cox regression analysis and machine‑learning approaches, we screened and determined that multiple proteins such as TMEM192 and POLM were prognostic factors associated with RFS, while multiple proteins such as GLYATL1 and TMEM109 were prognostic factors for OS
HostingRepository	iProX
AnnounceDate	2026-01-08
AnnouncementXML	Submission_2026-01-11_16:59:28.250.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guoli Deng
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-11 16:59:07	ID requested
⏵ 1	2026-01-11 16:59:28	announced

Dataset with its publication pending

submitter keyword: HepG2, Hep3B, mass spectrometry, membrane protein, HBV, proximity labeling

Shupeng Liu
contact affiliation	Shanghai Fourth People's Hospital
contact email	lshup@tongji.edu.cn
lab head
Guoli Deng
contact affiliation	Shanghai Fourth People's Hospital
contact email	guoli_deng@tongji.edu.cn
dataset submitter

iProX dataset URI