PXD072877-1

PXD072877 is an original dataset announced via ProteomeXchange.

Title	Abdominal-B regulates the male seminal fluid transferome and new female fecundity factors required for sperm sex peptide binding
Description	Seminal fluid proteins determine reproductive success in a wide range of animals. In the Drosophila male accessory gland, seminal fluid is mainly produced by two cell types, a vast majority of main cells and a small number of secondary cells that are characterized by a specialized secretory apparatus with unusually enlarged dense core granule vesicles. Loss of Abdominal-B expression from secondary cells in the enhancer mutant iab-6cocu disrupts their transcriptional and secretory identity. In consequence, mutant males fail to induce the long-term post-mating response, concomitant with loss of receptivity and sustained egg laying, in females. Here, we determine how secondary cells shape the seminal transferome and the female response by assessing iab-6cocu male accessory gland and female mate reproductive tract proteomes. We find downregulation of seminal fluid proteins constituting a signaling network that enables sperm binding and sustained action of key regulator Sex peptide and identify two new Sex peptide network proteins crucial for female fecundity, Cornutus (CG1701) and Hanrej (CG42564). Cornutus is required for dense core granule vesicle release, providing a link between the products of these compartments and the female long-term post-mating response. Our data highlights the importance of secondary cell signaling and secretion for overall seminal fluid composition and Sex peptide network function as well as the interdependence of main and secondary cells and their secretory products, advancing the general understanding of how seminal fluid signaling pathways modulate female physiology, sperm use and offspring production.
HostingRepository	PRIDE
AnnounceDate	2026-01-15
AnnouncementXML	Submission_2026-01-15_09:54:51.654.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Katarzyna Kjøge
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: NEWT:7227;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-09 23:59:51	ID requested
⏵ 1	2026-01-15 09:54:52	announced

10.64898/2026.01.10.698609;

submitter keyword: seminal fluid, transferome, Abdominal-B,Drosophila melanogaster

Jan Enghild
contact affiliation	Department of Molecular Biology and Genetics, Aarhus University, Denmark
contact email	jje@mbg.au.dk
lab head
Katarzyna Kjøge
contact affiliation	Aarhus University
contact email	kkjoege@mbg.au.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD072877
     1. Label: PRIDE project
     2. Name: Abdominal-B regulates the male seminal fluid transferome and new female fecundity factors required for sperm sex peptide binding