PXD072873-1

PXD072873 is an original dataset announced via ProteomeXchange.

Title	Discovery of a novel conserved gene family with an essential role in kinetoplastid parasite insect vector adhesion
Description	Leishmania adhesion to the sand fly stomodeal valve is important for parasite development and transmission, and occurs through its flagellum via a cytoskeletal adhesion complex. While three essential kinetoplastid-insect adhesion proteins (KIAPs) are known, the overall proteome of the adhesion complex is obscure. Using TurboID-tagged KIAP3 combined with proteomics and light microscopy, we have identified novel adhesion plaque components that localised to the adhered flagellum, including multiple members of the Adhesion Related p-loop NTPase Domain (ARND) family, of which the canonical member is the newly discovered KIAP4. Phylogenetic analysis of the ARND family showed it is conserved across the kinetoplastids, and we demonstrate that Trypanosoma congolense paralogs localise to the adhered flagellum in that parasite. Deletion of KIAP4 severely impaired haptomonad adhesion, with the mutant parasites unable to colonise the sand fly stomodeal valve. Our work provides details of the molecular machinery essential for vector colonisation and shows that these proteins have a conserved function in other kinetoplastid parasites.
HostingRepository	PRIDE
AnnounceDate	2026-06-26
AnnouncementXML	Submission_2026-06-26_04:09:49.392.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Barrack Owino
SpeciesList	scientific name: Leishmania mexicana MHOM/GT/2001/U1103; NCBI TaxID: NEWT:929439;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-09 18:36:58	ID requested
⏵ 1	2026-06-26 04:09:49	announced

10.1073/PNAS.2603653123;

submitter keyword: cytoskeleton, flagellum,Leishmania, adhesion, parasite-vector interactions

Jack D Sunter
contact affiliation	Oxford Brookes University, Department of Biological and Medical Sciences, Gipsy Lane, Oxford OX3 0BP, UK
contact email	jsunter@brookes.ac.uk
lab head
Barrack Owino
contact affiliation	Oxford Brookes University
contact email	19223175@brookes.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD072873
     1. Label: PRIDE project
     2. Name: Discovery of a novel conserved gene family with an essential role in kinetoplastid parasite insect vector adhesion