⮝ Full datasets listing
PXD072865-1
PXD072865 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | AX4 PPK1 Insoluble Dictyostelium proteomics, Scaglione Lab Duke |
| Description | Quantitative LC/MS/MS was performed on 2 uL of each sample, using a Vanquish Neo UPLC system (Thermo) coupled to a Thermo Orbitrap Astral high resolution accurate mass tandem mass spectrometer (Thermo). Briefly, the sample was first trapped on a Symmetry C18 20 mm x 180 um trapping column (5 ul/min at 99.9/0.1 v/v water/acetonitrile), after which the analytical separation was performed using a 1.5 um EvoSep 150um ID x 8cm performance (EvoSep) column with a 30 min gradient of 5 to 30% acetonitrile with 0.1% formic acid at a flow rate of 500 nanoliters/minute (nL/min) with a column temperature of 50C. Data collection on the Orbitrap Astral mass spectrometer was performed in a data-independent acquisition (DIA) mode of acquisition with a r=240,000 (m/z 200) full MS scan from m/z 380-980 with a target AGC value of 4e5 ions. Fixed DIA windows of 4 m/z from m/z 380 to 980 DIA MS/MS scans were acquired in the Astral with a target AGC value of 5e4 and max fill time of 6 ms. HCD collision energy setting of 27% was used for all MS2 scans. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-02-25 |
| AnnouncementXML | Submission_2026-02-25_09:00:17.293.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Erik Soderblom |
| SpeciesList | scientific name: Dictyostelium; NCBI TaxID: 5782; |
| ModificationList | Oxidation |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-01-09 13:29:21 | ID requested | |
| ⏵ 1 | 2026-02-25 09:00:17 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: label free, proteomics, DatasetType:Proteomics |
Contact List
| Matthew Scaglione | |
|---|---|
| contact affiliation | Duke University |
| contact email | matt.scaglione@duke.edu |
| lab head | |
| Erik Soderblom | |
| contact affiliation | Duke University |
| contact email | es114@duke.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000100428/ |
