PXD072854-1

PXD072854 is an original dataset announced via ProteomeXchange.

Title	Identification of the ubiquitination landscape in Arabidopsis upl3 upl4 mutants
Description	The ubiquitin-proteasome system (UPS) serves as the primary pathway regulating cellular homeostasis and environmental responses in eukaryotes. In this pathway, E3 ubiquitin ligases catalyse substrate ubiquitination, thereby marking targets for proteasomal degradation. UPL3 and UPL4 are proteasome-associated HECT-type ubiquitin ligases known to remodel substrate-attached ubiquitin chains at the proteasome, ensuring processive degradation. To investigate the role of UPL3/4 in regulating cellular ubiquitination, we enriched ubiquitinated proteins from wild-type and upl3 upl4 mutant seedlings using a Ubiquilin UBA domain tetramer. The purified ubiquitome was subsequently analysed by liquid chromatography-mass spectrometry (LC-MS).
HostingRepository	PRIDE
AnnounceDate	2026-08-06
AnnouncementXML	Submission_2026-08-05_23:41:38.742.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christos Spanos
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	ubiquitinylated lysine
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-09 09:01:51	ID requested
⏵ 1	2026-08-05 23:41:39	announced

Dataset with its publication pending

submitter keyword: Ubiquitin-proteasome sydtem (UPS), Lumos DDA, ubiquitome, ubiquitin ligases, UPL3, UPL4

Steven Spoel
contact affiliation	University of Edinburgh
contact email	Steven.Spoel@ed.ac.uk
lab head
Christos Spanos
contact affiliation	University of Edinburgh
contact email	spanos.christos@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD072854
     1. Label: PRIDE project
     2. Name: Identification of the ubiquitination landscape in Arabidopsis upl3 upl4 mutants