PXD072844-1

PXD072844 is an original dataset announced via ProteomeXchange.

Title	A deep origin of the RAF–SnRK2–PP2C functional module with distinctive ancestral features
Description	The SNF1-related protein kinase 2 (SnRK2) pathway is central to abiotic stress signaling in land plants; however, its evolutionary origin and ancestral functions remain poorly understood. Here, we identify and characterize a functional SnRK2 signaling module in the unicellular green alga Chlamydomonas reinhardtii. We demonstrate that the algal isoform SnRK2.7 is essential for osmotic stress tolerance, providing a pre- abscisic acid (ABA) model system for characterizing SnRK2 signaling. Functional analysis reveals that SnRK2.7 exhibits key regulatory elements conserved in land plant SnRK2s, but also displays lineage-specific features: it is required for general cellular viability in algae and localizes to the contractile vacuole—an osmoregulatory organelle lost during streptophyte evolution. Notably, SnRK2.7 phosphorylates PP2C3, a clade A protein phosphatase, revealing an ancestral regulatory logic distinct from that in angiosperms, where PP2Cs functions upstream, dephosphorylating SnRK2 proteins. Together with MAPKKK3, a B1/B3-RAF kinase, these proteins form an evolutionarily ancient SnRK2 signaling module. Our findings reveal that a sophisticated SnRK2 pathway predates both the evolution of ABA signaling and plant terrestrialization, implying key regulatory innovations that emerged during chlorophyte-streptophyte divergence.
HostingRepository	PRIDE
AnnounceDate	2026-07-02
AnnouncementXML	Submission_2026-07-02_02:01:05.331.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	GKN JK
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: NEWT:3055;
ModificationList	phosphorylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-09 04:50:38	ID requested
⏵ 1	2026-07-02 02:01:06	announced

10.1111/NPH.71438;

submitter keyword: RAF kinase, cell survival, clade A PP2C,SnRK2, Chlamydomonas reinhardtii

Xiaobo Li
contact affiliation	Associate professer
contact email	lixiaobo@westlake.edu.cn
lab head
GKN JK
contact affiliation	Westlake University
contact email	guokangning@westlake.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD072844
     1. Label: PRIDE project
     2. Name: A deep origin of the RAF–SnRK2–PP2C functional module with distinctive ancestral features