⮝ Full datasets listing
PXD072808-1
PXD072808 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Oxidative stress causes a reversible decrease of deubiquitylases activity in old vertebrate brains - AQUA |
| Description | The ubiquitin-proteasome system is essential for neuronal proteostasis, and its activity declines with age. How deubiquitylating enzymes (DUBs) are affected by aging in the vertebrate brain remains unclear. Here, we profiled cysteine protease DUBs using activity-based proteomics in aging mice and killifish brains. We identified a subset of DUBs that progressively lose catalytic activity with age, despite stable protein levels. We demonstrated that oxidative stress impairs DUB function through thiol oxidation and that antioxidant treatment by N-acetylcysteine ethyl ester (NACET) restores their activity in vitro and in vivo. Moreover, global DUB inhibition as well as targeted inhibition of USP7, one of the DUBs most strongly affected during aging, in human iPSC-derived neurons partially recapitulated ubiquitylation changes observed in aged brains. Temporal analysis in mice further revealed that DUB inhibition precedes proteasome decline in the brain during aging. Together, these findings indicate a redox-sensitive subset of DUBs that undergo an age-associated decline in activity and suggest that impaired deubiquitylation is an early, yet potentially reversible, driver of proteostasis decline in the aging brain. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-03-04 |
| AnnouncementXML | Submission_2026-03-04_04:27:44.619.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Leibniz FLI Proteomics |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | Oxidation; Acetyl |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-01-08 02:12:06 | ID requested | |
| ⏵ 1 | 2026-03-04 04:27:45 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Brain aging, Deubiquitylases (DUBs), Activity-based proteomics, Oxidative stress, Ubiquitin-Proteasome System (UPS), USP7, N-acetylcysteine ethyl ester (NACET), DatasetType:Proteomics |
Contact List
| Alessandro Ori | |
|---|---|
| contact affiliation | Leibniz Institute on Aging Fritz Lipmann Institute (FLI) |
| contact email | alessandro.ori@leibniz-fli.de |
| lab head | |
| Leibniz FLI Proteomics | |
| contact affiliation | Leibniz FLI |
| contact email | proteomics@leibniz-fli.de |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000100404/ |
