PXD072752-1

PXD072752 is an original dataset announced via ProteomeXchange.

Title	Polianthes tuberosa L. Extract Suppresses Melanogenesis through Concurrent Inhibition of cAMP/CREB and MAPK Signaling Pathways
Description	Ultraviolet (UV) radiation is a primary environmental stimulus for skin hyperpigmentation. Polianthes tuberosa L. (PT), rich in polyphenols and flavonoids, possesses antioxidant and anti-inflammatory properties, yet the anti-melanogenic mechanism of the PT extract (PTE) remains unexplored. Network pharmacology revealed nuclear factor erythroid-derived 2-like 2 (NFE2L2), superoxide dismutase 1 (SOD1), nuclear factor kappa B subunit 1 (NFKB1) and interleukin-6 (IL-6) as core targets. This suggests that the therapeutic mechanism of the PTE may involve the coordinated modulation of oxidative stress (via NFE2L2/SOD1) and inflammation (via NFKB1/IL-6)—two interconnected pathways that critically drive melanogenesis following UV exposure. The PTE dose-dependently inhibited UV-induced release of inflammatory cytokines and paracrine melanogenic factors in the immortalized human keratinocyte cell line. It also mitigated UV-induced collagen reduction in fibroblasts. In the mouse melanoma cell line B16F10, the PTE significantly suppressed melanogenesis and tyrosinase activity (p < 0.001). Integration of transcriptomic and proteomic data provided a complementary view of molecular regulation at both the messenger RNA and protein levels. This convergent evidence indicated that PTE acts as a concurrent inhibitor of the cyclic adenosine monophosphate (cAMP)/protein kinase A/cAMP-response element binding protein and mitogen-activated protein kinase signaling axes, as validated by quantitative polymerase chain reaction and western blot analyses. This dual inhibition led to the downregulation of microphthalmia-associated transcription factor and its downstream melanogenic enzymes. Our findings underscore the potential of PTE as a multifaceted, natural whitening agent for cosmetic applications.
HostingRepository	PRIDE
AnnounceDate	2026-02-16
AnnouncementXML	Submission_2026-02-15_16:27:41.971.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qiaozhen Li
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-07 04:09:14	ID requested
⏵ 1	2026-02-15 16:27:42	announced

10.1038/s41598-026-36962-9;

Li Q, Zhu H, Jiang T, Hou R, Li J, Yan X, Wang J, Polianthes tuberosa L. Extract suppresses melanogenesis through concurrent Inhibition of cAMP/CREB and MAPK signaling pathways. Sci Rep, 16(1):6137(2026) [pubmed]

submitter keyword: Anti-melanogenesis

Hyperpigmentation

Mechanistic study

Oxidative stress

Polianthes tuberosa L. flower

Qiaozhen Li
contact affiliation	School of Chemical and Material Engineering, Jiangnan University
contact email	18459379609@163.com
lab head
Qiaozhen Li
contact affiliation	Jiangnan University
contact email	18459379609@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD072752

PRIDE project URI

• PRIDE
  1. PXD072752
     1. Label: PRIDE project
     2. Name: Polianthes tuberosa L. Extract Suppresses Melanogenesis through Concurrent Inhibition of cAMP/CREB and MAPK Signaling Pathways