PXD072749 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Functional characterisation of components in two Plasmodium falciparum Cullin-RING-Ligase complexes |
| Description | Ubiquitination is the key eukaryotic post-translational modification that governs protein degradation, localisation, and activity which is mediated by a concerted enzyme cascade. The largest superfamily of these enzymes include the Cullin-RING-Ligase (CRL) complexes. Plasmodium falciparum, the causative agent of the most severe form of malaria in humans, encodes the critical proteins required for ubiquitination, but we do not yet understand the function of this pathway. Here the P. falciparum CRL complexes were characterised to reveal an essential but minimal repertoire controlled by two Cullin scaffolds. A PfCullin1-linked CRL complex, recruiting a single substrate receptor, was identified as being required for parasite inner-membrane biogenesis and DNA replication. A second CRL complex functioning through a PfCullin4 scaffold was identified that utilised a previously unidentified adaptor protein and receptors to support DNA replication. These results show that the P. falciparum CRL complexes are essential in both nuclear maintenance and membrane integrity. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-01-09 |
| AnnouncementXML | Submission_2026-01-09_01:51:46.988.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Laura Dagley |
| SpeciesList | scientific name: Plasmodium falciparum (isolate 3D7); NCBI TaxID: NEWT:36329; |
| ModificationList | phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Eclipse; timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-01-07 02:41:54 | ID requested | |
| ⏵ 1 | 2026-01-09 01:51:47 | announced | |
Publication List
| 10.1038/s41598-025-05342-0; |
| 10.1038/S41598-025-05342-0; |
| Marapana D, Cobbold SA, Pasternak M, Shami GJ, Ralph SA, Lopaticki S, Yousef J, Vaibhav V, Dagley LF, Komander D, Cowman AF, Functional characterisation of components in two Plasmodium falciparum Cullin-RING-Ligase complexes. Sci Rep, 15(1):21359(2025) [pubmed] |
Keyword List
Contact List
| Alan Cowman |
|---|
| contact affiliation | The Walter and Eliza Hall institute of Medical Research, Department of Medical Biology, Faculty of Medicine, Dentistry and Health Sciences, The University of Melbourne |
| contact email | cowman@wehi.edu.au |
| lab head | |
| Laura Dagley |
|---|
| contact affiliation | WEHI |
| contact email | dagley.l@wehi.edu.au |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD072749 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD072749
- Label: PRIDE project
- Name: Functional characterisation of components in two Plasmodium falciparum Cullin-RING-Ligase complexes
