PXD072715 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Chaperone-mediated autophagy is required for regulatory T cell function |
| Description | Chaperone-mediated autophagy (CMA) is a selective form of protein degradation in lysosomes that declines with age. Besides protein quality control, CMA also regulates several cellular processes through timely proteome remodeling. We previously demonstrated the importance of CMA in the activation of helper T cells. In this work, we analyzed the role of CMA in the generation and function of regulatory T cells (Tregs), a specialized type of T cells that suppress immune responses. We found that the basal CMA activity of Tregs further increases upon their activation. Using a Treg-specific CMA-deficient mouse model, we show that CMA is crucial for maintenance of peripheral tolerance by Tregs. Mice with CMA-defective Tregs display signs of chronic inflammation, which results in reduced survival as they age. We demonstrate that CMA-deficient Tregs have reduced suppressive activity in vivo using an experimental model of inflammatory bowel disease and a second model of tumor-induced immune response. Comparative quantitative proteomic analysis enabled us to identify the subproteome degraded by CMA and, consequently, the cellular pathways modulated by this type of autophagy to sustain Treg homeostasis and function. Collectively, our findings uncover a previously unknown role for CMA in regulating Treg function. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-21 |
| AnnouncementXML | Submission_2026-04-21_08:38:59.581.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Simone Sidoli |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF HT |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-01-06 12:31:51 | ID requested | |
| ⏵ 1 | 2026-04-21 08:39:00 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Autophagy |
| Chronic Inflammation |
| Inflammaging |
| Immunotolerance |
| Lysosomes |
| Proteostasis |
Contact List
| Simone Sidoli |
|---|
| contact affiliation | Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, United States |
| contact email | simone.sidoli@einsteinmed.edu |
| lab head | |
| Simone Sidoli |
|---|
| contact affiliation | Albert Einstein College of Medicine |
| contact email | simone.sidoli@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD072715 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD072715
- Label: PRIDE project
- Name: Chaperone-mediated autophagy is required for regulatory T cell function
