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PXD072688-1
PXD072688 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Fe(III) Mineral Encrustation Uncouples Respiration from Reproduction of the Nitrate-Reducing Fe(II)-Oxidizing bacterium Acidovorax sp. BoFeN1 |
| Description | Fe(II) can be oxidized by mixotrophic nitrate-reducing Fe(II)-oxidizing bacteria to Fe(III), leading to mineral precipitation. However, the effects of this process on the metabolism and reproduction ability of the Fe(II)-oxidizing bacterial cells have not yet been quantified. In this study, we investigated the effects of Fe(II) on the mixotrophic nitrate-reducing Fe(II)-oxidizing bacteria Acidovorax sp. BoFeN1 by performing cell plate counts, chemical analyses, and scanning electron microscopy (SEM) imaging. The result showed that nitrate-reducing Fe(II)-oxidizing bacteria Acidovorax sp. BoFeN1 undergoes a striking physiological state in which respiration persists while cell reproduction collapses after their oxidation of Fe(II). Across 0.1–10 mM Fe(II), colony-forming units dropped by up to 103-fold, yet acetate consumption remained largely unaffected and nitrate reduction was even slightly promoted. SEM-EDS reveals extensive Fe(III) encrustation on cells. Adding an Fe(III) chelator, citrate, restored reproductive capacity without notably changes in Fe(II) oxidation or nitrate reduction, indicating that it was the products of Fe(II) oxidation, the secondary Fe(III) minerals, that impaired cell reproduction. These findings uncover a mineral encrustation-driven uncoupling between respiration and reproduction, suggest how natural organic ligands may buffer NRFeOx bacterial communities in Fe-rich anoxic environments, and point to cellular-level levers for mitigating denitrification failures and N2O risks in engineered and natural systems. |
| HostingRepository | iProX |
| AnnounceDate | 2026-01-01 |
| AnnouncementXML | Submission_2026-01-05_19:40:20.259.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Heping Zhang |
| SpeciesList | scientific name: Acidovorax; NCBI TaxID: 12916; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF HT |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-01-05 19:38:32 | ID requested | |
| ⏵ 1 | 2026-01-05 19:40:20 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Fe(II), reproductive capacity, iron oxidation, nitrate reduction, metabolism |
Contact List
| Chao Peng | |
|---|---|
| contact affiliation | China West Normal University |
| contact email | chpeng89@cwnu.edu.cn |
| lab head | |
| Heping Zhang | |
| contact affiliation | China West Normal University |
| contact email | 1664927877@qq.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
