PXD072667 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Toxicoproteomics of HepG2 Reveals Translational Biomarkers of Paracetamol-Induced Liver Injury and Recovery |
| Description | Drug-induced liver injury (DILI) is a leading cause of acute liver failure (ALF) and a major obstacle in drug development, reflecting the limited predictive value of animal models and lack of robust,mechanism-based biomarkers. Human in vitro systems, coupled with unbiased omics approaches, have emerged as a potential alternative strategy. Here, quantitative toxicoproteomics was applied to paracetamol (acetaminophen; APAP)-exposed HepG2 cells to identify translational biomarkers of APAP-induced DILI. Cells were exposed to the IC₅₀ concentration of APAP for 8 h (injury phase), followed by drug withdrawal and a 24 h recovery phase. LC–MS/MS analysis quantified 1,478 proteins and revealed increased protein secretion upon APAP exposure. Notably, comparison of the proteomic dataset against available enriched proteomic profiles of ALF patients’ serum confirmed the clinical relevance of the molecular signature obtained. Injury-phase upregulated proteins, including KRT18 and HMGB1, recapitulated known clinical DILI biomarkers. Conversely, recovery was characterized by enrichment of the liver regeneration markers AFP, APOA1 and HP, enabling discrimination between injury and regeneration. HPD, S100A6 and TXNRD1 emerged as candidate diagnostic biomarkers, while SERPINA1 and complement components (C3, C5) showed prognostic potential. Collectively, this study demonstrates that toxicoproteomics applied to human in vitro hepatic models, validated against patient-derived data, enables the discovery of novel translational biomarkers of liver injury and recovery. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-07-27 |
| AnnouncementXML | Submission_2026-07-27_02:42:37.239.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Rui Vitorino |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | amidated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-01-05 12:16:16 | ID requested | |
| ⏵ 1 | 2026-07-27 02:42:37 | announced | |
Publication List
Keyword List
Contact List
| Joana Miranda |
|---|
| contact affiliation | Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Lisbon, Portugal rvitorino@ua.pt iBiMED- Department of Medical Siences University of Aveiro |
| contact email | jmiranda@ff.ulisboa.pt |
| lab head | |
| Rui Vitorino |
|---|
| contact affiliation | Department of Medical Sciences, Institute of Biomedicine iBiMED, University of Aveiro, Aveiro, Portugal |
| contact email | ruimpvitorino@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD072667
- Label: PRIDE project
- Name: Toxicoproteomics of HepG2 Reveals Translational Biomarkers of Paracetamol-Induced Liver Injury and Recovery
