PXD072616-1

PXD072616 is an original dataset announced via ProteomeXchange.

Title	Gadd45a Knockout Alleviates Cisplatin-Induced Hearing Loss by Inhibiting CXCL Family Protein Expression
Description	Gadd45a knockout inhibits cisplatin-induced cell death in the OC1 cell line. Transcriptome sequencing revealed that Gadd45a knockout suppresses the expression of CXCL family cytokines. This finding was further validated by protein-sequencing of the cell culture supernatant. Mechanistically, Gadd45a knockout specifically inhibits NF-κB1 (p105/p50) but not RELA (p65), both of which are transcription factors for CXCL family genes. Immunofluorescence staining demonstrated that Gadd45a knockout inhibits the nuclear translocation of NF-κB1 protein, but not that of RELA. In vivo, both si-Gadd45a injection into the semicircular canal and conditional knockout of Gadd45a effectively attenuated cisplatin-induced ototoxicity.
HostingRepository	iProX
AnnounceDate	2026-01-03
AnnouncementXML	Submission_2026-01-03_23:29:45.340.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Weilong Wang
SpeciesList	scientific name: Rattus norvegicus; NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-03 23:29:30	ID requested
⏵ 1	2026-01-03 23:29:45	announced

Dataset with its publication pending

submitter keyword: Ototoxicity, Cisplatin, Gadd45a, NF-κB1(p105/p50), CXCL family

Zuhong He
contact affiliation	Department of Otorhinolaryngology-Head and Neck Surgery, Zhongnan Hospital of Wuhan University
contact email	hezuhong@163.com
lab head
Weilong Wang
contact affiliation	Department of Otorhinolaryngology-Head and Neck Surgery, Zhongnan Hospital of Wuhan University
contact email	wwlgoodluck@163.com
dataset submitter

iProX dataset URI