⮝ Full datasets listing
PXD072609-2
PXD072609 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Gelatin/lignin hydrogel loaded with mesenchymal stem cell-derived exosomes enriched in microRNA-185 inhibits progression of oral cancer |
| Description | Purpose: Due to the lack of effective local therapeutic strategies for oral squamous cell carcinoma (OSCC), this study aimed to develop a novel gelatin/lignin hydrogel loaded with mesenchymal stem cell (MSC) derived exosomes enriched in microRNA-185 (miR-185 EV) for intraoral delivery, followed by systematic evaluation of its therapeutic efficacy and underlying molecular mechanisms. Materials and Methods: The gelatin/lignin hydrogel was prepared and subsequently loaded with miR-185 EV. The physicochemical properties of the hydrogel, including microstructure, swelling behavior, chemical composition, and rheological characteristics were systematically evaluated. Next, the stability, viscosity, biocompatibility, and exosome release kinetics of the hydrogel were further assessed. A 4-nitroquinoline-1-oxide (4NQO) induced mouse tongue carcinogenesis model was established to assess the in vivo antitumor activity of the hydrogel via intraoral administration. Moreover, proteomic analysis was conducted to investigate the molecular mechanisms of miR-185 EV on OSCC. Results: The miR-185 EV-loaded gelatin/lignin hydrogel exhibited favorable physicochemical properties, stability, and biocompatibility, while prolonging the tissue retention time of miR-185 EV. In vivo antitumor efficacy experiments showed that the miR-185 EV-loaded hydrogel significantly inhibited tumor occurrence and alleviated epithelial dysplasia. Immunohistochemical analyses revealed significant suppression of tumor proliferation and epithelial–mesenchymal transition (EMT) of the hydrogel. Proteomic analysis indicated that miR-185 EV suppressed OSCC progression by downregulating interleukin-1β (IL-1β), consequently inhibiting the NF-κB signaling pathway. Conclusion: The findings demonstrate the successful development of the miR-185 EV-loaded gelatin/lignin hydrogel that represents an effective nanomedicine platform for intraoral drug delivery, providing a promising strategy for the clinical treatment of OSCC. |
| HostingRepository | iProX |
| AnnounceDate | 2025-12-31 |
| AnnouncementXML | Submission_2026-01-22_00:27:50.379.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Meitong Liu |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF HT |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-01-03 22:55:22 | ID requested | |
| 1 | 2026-01-03 23:24:48 | announced | |
| ⏵ 2 | 2026-01-22 00:27:50 | announced | 2026-01-22: Update info |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: hydrogel, MSC-derived EVs, microRNA-185, OSCC, drug delivery |
Contact List
| Xiaobing Guan | |
|---|---|
| contact affiliation | School of Stomatology, Capital Medical University |
| contact email | guanxbing2013@163.com |
| lab head | |
| Meitong Liu | |
| contact affiliation | School of Stomatology, Capital Medical University |
| contact email | Aryalmtong@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
