PXD072601-1

PXD072601 is an original dataset announced via ProteomeXchange.

Title	Targeting peptidyl-arginine deiminase 4 suppresses SARS-CoV-2 replication and modulates the inflammatory response
Description	This project presents a comprehensive, quantitative proteomics and citrullinome profiling study designed to define how viral infection remodels host protein citrullination and related cellular pathways. The experimental design integrates in vitro and in vivo models of infection, including cultured human cell lines and infected murine tissues, with parallel mock-treated or vehicle controls to enable robust comparative analyses. Proteins were labeled using a citrulline-specific chemical probe, enriched by streptavidin-based affinity purification, and processed through on-bead digestion followed by isobaric tag–based quantitative mass spectrometry to allow multiplexed comparison across biological conditions and replicates. This strategy enabled systematic identification and relative quantification of citrullinated host and viral proteins, alongside global proteome changes, while controlling for technical variability through standardized normalization and statistical workflows. The resulting dataset captures condition-specific alterations in protein modification, abundance, and functional pathways, providing a well-controlled resource for interrogating the role of post-translational modifications in viral replication, host inflammatory responses, and disease-associated molecular mechanisms.
HostingRepository	PRIDE
AnnounceDate	2026-01-30
AnnouncementXML	Submission_2026-01-30_09:01:44.013.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD072601
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Webb Camille
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-03 12:08:36	ID requested
⏵ 1	2026-01-30 09:01:44	announced

10.6019/PXD072601;

submitter keyword: Protein arginine deiminase, inflammation, COVID, lung,PADI4

Paul Thompson
contact affiliation	University of Massachusetts Chan Medical School
contact email	paul.thompson@umassmed.edu
lab head
Webb Camille
contact affiliation	University of Massachusetts Chan Medical School
contact email	webb.camille@umassmed.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD072601

PRIDE project URI

• PRIDE
  1. PXD072601
     1. Label: PRIDE project
     2. Name: Targeting peptidyl-arginine deiminase 4 suppresses SARS-CoV-2 replication and modulates the inflammatory response