PXD072564 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Bioorthogonal Click Chemistry for Antibody-Free Profiling of Acetylation, Propionylation, and Butyrylation in Pseudomonas aeruginosa and Methicillin-Resistant Staphylococcus aureus |
| Description | Lysine acylation is a post-translational modification (PTM) conserved in all domains of life and is essential for regulating diverse biological processes. Traditional methods for investigating acylation rely on anti-acyl-lysine-antibodies, which are costly, time-consuming, and often exhibit variable affinity. To remedy these pitfalls, we developed an antibody-free method for bacterial acylome enrichment using bioorthogonal click chemistry coupled with tandem mass spectrometry. We applied this approach to the pathogens Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus (MRSA) to understand the regulatory role of acylation in each organism. We characterized the acetylome, propionylome and butyrylome in P. aeruginosa UCBPP-PA14 and the acetylome and propionylome in MRSA. Comparative analyses revealed distinct PTM dynamics showing that acylation regulated a wide range of cellular functions, including metabolism, antibiotic resistance, virulence, and stress response. This work establishes the first antibody-free enrichment method for defining bacterial acylomes and provides new insight into global lysine acylation networks in pathogenic bacteria. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-11 |
| AnnouncementXML | Submission_2026-06-11_10:17:26.320.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Christian Montes Serey |
| SpeciesList | scientific name: Pseudomonas aeruginosa (strain UCBPP-PA14); NCBI TaxID: NEWT:208963; scientific name: Staphylococcus aureus; NCBI TaxID: NEWT:1280; |
| ModificationList | acetylated residue; propanoylated residue; butanoylated residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-12-31 16:27:40 | ID requested | |
| ⏵ 1 | 2026-06-11 10:17:27 | announced | |
Publication List
Keyword List
| submitter keyword: Butyrylation, Propionylation, Posttranslational Modifications, Acetylation,Bacterial Acylation |
Contact List
| Justin Walley |
|---|
| contact affiliation | Department of Plant Pathology, Entomology, and Microbiology Iowa State University |
| contact email | jwalley@iastate.edu |
| lab head | |
| Christian Montes Serey |
|---|
| contact affiliation | Iowa State University |
| contact email | chrisfm@iastate.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD072564
- Label: PRIDE project
- Name: Bioorthogonal Click Chemistry for Antibody-Free Profiling of Acetylation, Propionylation, and Butyrylation in Pseudomonas aeruginosa and Methicillin-Resistant Staphylococcus aureus
