PXD072529-1

PXD072529 is an original dataset announced via ProteomeXchange.

Title	VIPP1 and VIA1 interactome AP-MS
Description	Thylakoid membranes are highly dynamic structures that are essential for photosynthesis and particularly vulnerable to photo-oxidative stress. Although several factors involved in thylakoid biogenesis and maintenance have been identified, the molecular mechanisms that preserve membrane integrity under stress conditions remain poorly understood. In this study, we used mass spectrometry–based proteomics to identify protein complexes involved in chloroplast membrane stress responses. Guided by the chloroplast unfolded protein response (cpUPR), a stress signaling pathway that links chloroplast proteostasis to membrane remodeling, we focused on proteins upregulated in a MARS1-dependent manner during photo-oxidative stress in Chlamydomonas reinhardtii. FLAG immunoprecipitation coupled to mass spectrometry (IP–MS) was employed to characterize the interactome of VIA1 (VIPP1-Associated Protein 1), a conserved chloroplast protein identified as a cpUPR target, and to define its relationship with the thylakoid membrane remodeling factor VIPP1. A reciprocal IP–MS experiment using a polyclonal VIPP1 antibody was performed under control and high-light stress conditions to identify stress-dependent interactions and confirm the binding to VIA1. An additional VIPP1-IP experiment was conducted to assess the impact of targeted mutations that disrupt the VIA1–VIPP1 interaction. To evaluate evolutionary conservation, complementary FLAG-based IP–MS analyses were performed in Synechocystis sp. PCC 6803 expressing a tagged VIA1 ortholog. Together, these datasets define conserved stress-associated interaction networks centered on VIA1 and VIPP1 and provide proteomic evidence for a conserved membrane stress–response module operating across photosynthetic organisms.
HostingRepository	PRIDE
AnnounceDate	2026-08-20
AnnouncementXML	Submission_2026-08-20_11:41:42.223.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Imre
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: NEWT:3055; scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: NEWT:1148;
ModificationList	phosphorylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Astral; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-30 13:49:03	ID requested
⏵ 1	2026-08-20 11:41:43	announced

Vetrano P, Krall K, Martinez L, Traverso E, Morosinotto T, Irwin NAT, Mazor Y, Ramundo S, VIA1 is a conserved regulator of thylakoid membrane integrity that acts through VIPP1. Proc Natl Acad Sci U S A, 123(34):e2605816123(2026) [pubmed]

10.1073/pnas.2605816123;

submitter keyword: Chlamydomonas reinhardtii, VIPP1, VIA1, chloroplast, thylakoids

Silvia Ramundo
contact affiliation	Gregor Mendel Institute of Molecular Plant Biology, Vienna BioCenter, Vienna, Austria
contact email	Silvia.ramundo@gmi.oeaw.ac.at
lab head
Richard Imre
contact affiliation	IMBA Vienna
contact email	richard.imre@imba.oeaw.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD072529

PRIDE project URI

• PRIDE
  1. PXD072529
     1. Label: PRIDE project
     2. Name: VIPP1 and VIA1 interactome AP-MS